PMID- 9689932
OWN - NLM
STAT- MEDLINE
DCOM- 19980814
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 105
IP  - 1
DP  - 1998 Aug
TI  - Molecular definition of chromosome translocations involving 10q24 and 19q13 in 
      human malignant glioma cells.
PG  - 60-8
AB  - Loss of heterozygosity (LOH) analysis has repeatedly implicated the 10q24-26 
      region as the site of tumor suppressor genes involved in the development of 
      malignant human gliomas. However, deletions of this kind are generally too big to 
      pinpoint the critical genes involved. On the other hand, chromosome 
      translocations frequently interrupt genes important in the development of the 
      phenotype. We have screened a series of cell lines and cultures from primary 
      human brain tumors for translocations involving chromosomes 10 and 19 by using 
      fluorescence in situ hybridization (FISH) and chromosome-specific paints. The 
      T98G cell line carries an apparently reciprocal t(10;19)(q24;q13) translocation, 
      the breakpoints on chromosomes 10 and 19 occurring in a region frequently showing 
      LOH in gliomas as well as oligodendrogliomas and astrocytomas. One glioblastoma 
      tumor, CCF 4, also showed a subtle translocation of chromosome 10 material into 
      the long arm of chromosome 11. FISH analysis of these rearrangements showed that 
      the chromosome 10-specific yeast artificial chromosome (YAC) 912C7 spans the 
      translocation breakpoint in T98G cells and is also present in the material 
      translocated from chromosome 10 in tumor CCF 4. The translocation breakpoint in 
      19q13 in T98G occurs within a 500-kb region of YAC 751E12. These translocation 
      breakpoints are within the regions showing frequent LOH in brain tumors and 
      provide a more refined tool for the identification of genes in this region 
      involved in tumorigenesis.
FAU - Chernova, O
AU  - Chernova O
AD  - Department of Neurosciences, Lerner Research Institute, Cleveland Clinic 
      Foundation, Ohio, USA.
FAU - Cowell, J K
AU  - Cowell JK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Brain Neoplasms/*genetics/pathology
MH  - Chromosome Breakage/genetics
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - Clone Cells
MH  - Gene Rearrangement
MH  - Glioma/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1998/08/05 00:00
MHDA- 1998/08/05 00:01
CRDT- 1998/08/05 00:00
PHST- 1998/08/05 00:00 [pubmed]
PHST- 1998/08/05 00:01 [medline]
PHST- 1998/08/05 00:00 [entrez]
AID - S0165460897004792 [pii]
AID - 10.1016/s0165-4608(97)00479-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Aug;105(1):60-8. doi: 10.1016/s0165-4608(97)00479-2.