PMID- 9690131
OWN - NLM
STAT- MEDLINE
DCOM- 19981109
LR  - 20180713
IS  - 0213-3911 (Print)
IS  - 0213-3911 (Linking)
VI  - 13
IP  - 3
DP  - 1998 Jul
TI  - Lectin binding in the human endometrium in early luteal phase following 
      controlled ovarian hyperstimulation.
PG  - 737-42
LID - 10.14670/HH-13.737 [doi]
AB  - A lectin histochemical study was performed to investigate the glycoconjugate 
      saccharidic moieties on the endometrial epithelium and stroma in 12 women 
      undergoing controlled ovarian hyperstimulation (COH) for in-vitro fertilisation 
      for embryo transfer (IVF-ET) in early luteal phase. 7 control subjects were also 
      evaluated. For this purpose a battery of seven horseradish peroxidase-conjugated 
      lectins (PNA, SBA, DBA, WGA, ConA, LTA and UEA I) was used. Cytochemical controls 
      were performed for specificity of lectin-sugar reaction. As far as the 
      endometrial glands and stroma are concerned, the obtained data showed no 
      differences in the endometrial lectin binding between the subjects of the control 
      group and the ones undergoing COH, with the exception of PNA reactivity at the 
      level of the apical portion of the glandular cells, which was detected only in 
      COH women. It is noteworthy that, although the endometrial dating using the 
      Noyes's criteria showed marked dissynchronies between the stroma and the glands 
      in COH subjects, a uniformity of lectin binding, revealing the same type and 
      localization of terminal oligosaccharides, was observed in all the examined 
      subjects. The uniformity in distribution of the sugar residues detected in the 
      endometrial specimens following COH might be due to the massive FSH and/or hCG 
      treatment which probably determines an endometrial environment almost equal in 
      all the examined subjects. In all the treated subjects reactivity with 
      sialidase-WGA and ConA, revealing the presence of N-acetyl-D-glucosamine and 
      D-mannose respectively, was detected at the level of the lining epithelium.
FAU - Gheri, G
AU  - Gheri G
AD  - Department of Human Anatomy and Histology, University of Florence, Italy.
FAU - Bryk, S G
AU  - Bryk SG
FAU - Taddei, G
AU  - Taddei G
FAU - Borri, P
AU  - Borri P
FAU - Noci, I
AU  - Noci I
LA  - eng
PT  - Journal Article
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (Lectins)
RN  - 0 (Peanut Agglutinin)
RN  - 0 (Plant Lectins)
RN  - 0 (Soybean Proteins)
RN  - 0 (Ulex europaeus lectins)
RN  - 0 (Wheat Germ Agglutinins)
RN  - 0 (dolichos biflorus agglutinin)
RN  - 0 (fucose-binding lectin)
RN  - 0 (soybean lectin)
RN  - 11028-71-0 (Concanavalin A)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Adult
MH  - Concanavalin A/metabolism
MH  - Endometrium/*metabolism/pathology
MH  - Female
MH  - Fertilization in Vitro
MH  - Horseradish Peroxidase/metabolism
MH  - Humans
MH  - Lectins/*metabolism
MH  - Ovarian Hyperstimulation Syndrome/chemically induced/metabolism
MH  - Ovary
MH  - Peanut Agglutinin/metabolism
MH  - *Plant Lectins
MH  - *Soybean Proteins
MH  - Wheat Germ Agglutinins/metabolism
EDAT- 1998/08/05 00:00
MHDA- 1998/08/05 00:01
CRDT- 1998/08/05 00:00
PHST- 1998/08/05 00:00 [pubmed]
PHST- 1998/08/05 00:01 [medline]
PHST- 1998/08/05 00:00 [entrez]
AID - 10.14670/HH-13.737 [doi]
PST - ppublish
SO  - Histol Histopathol. 1998 Jul;13(3):737-42. doi: 10.14670/HH-13.737.