PMID- 9690532
OWN - NLM
STAT- MEDLINE
DCOM- 19980813
LR  - 20061115
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 83
IP  - 3
DP  - 1998 Aug 1
TI  - Intratumor distribution of 1p deletions in human colorectal adenocarcinoma is 
      commonly homogeneous: indirect evidence of early involvement in colorectal 
      tumorigenesis.
PG  - 415-22
AB  - BACKGROUND: Cytogenetics and molecular biology studies have indicated that a 
      large subset of human colorectal adenocarcinomas have distal 1p chromosome arm 
      deletions. The aim of this study was to evaluate the intratumor distribution of 
      1p deletions under the assumption that homogeneity is an indication of early 
      occurrence. METHODS: Seventy-nine histologically selected primary sectors (40 
      superficial and 39 deep) and 3 lymph node metastases obtained from 20 human 
      sporadic adenocarcinomas were analyzed. Interphase two-color fluorescence in situ 
      hybridization (FISH) was applied to cytocentrifuged nuclei using a centromeric 
      probe for chromosome 1 and a telomeric probe mapping to the 1p36 band. RESULTS: 
      Deletions at 1p were observed in 35 of 82 tumor samples corresponding to 9 of 20 
      adenocarcinomas analyzed (45%). Seven of the 9 adenocarcinomas with 1p deletions 
      showed an intratumor presence of these aberrations in all the different tumor 
      sectors. CONCLUSIONS: These data, acquired by FISH interphase cytogenetics, 
      confirm that 1p deletions in colorectal adenocarcinoma are common and suggest 
      that this structural chromosomal aberration occurs mainly as an early event in 
      colorectal tumorigenesis.
FAU - Di Vinci, A
AU  - Di Vinci A
AD  - Laboratory of Biophysics and Cytometry, National Institute for Cancer Research, 
      Genoa, Italy.
FAU - Infusini, E
AU  - Infusini E
FAU - Nigro, S
AU  - Nigro S
FAU - Monaco, R
AU  - Monaco R
FAU - Giaretti, W
AU  - Giaretti W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adenocarcinoma/etiology/*genetics
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 1
MH  - Colorectal Neoplasms/etiology/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
EDAT- 1998/08/05 02:04
MHDA- 2000/06/20 09:00
CRDT- 1998/08/05 02:04
PHST- 1998/08/05 02:04 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/08/05 02:04 [entrez]
AID - 10.1002/(SICI)1097-0142(19980801)83:3<415::AID-CNCR8>3.0.CO;2-N [pii]
PST - ppublish
SO  - Cancer. 1998 Aug 1;83(3):415-22.