PMID- 9690678
OWN - NLM
STAT- MEDLINE
DCOM- 19980814
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 73
IP  - 6
DP  - 1998 Jun
TI  - FISH-based analysis of stable translocations in a Techa River population.
PG  - 605-12
AB  - PURPOSE: Measurements of symmetrical translocations by fluorescence in situ 
      hybridization (FISH) were performed for retrospective biodosimetry in a Techa 
      River population exposed to external (gamma-rays) and internal (90Sr, 137Cs) 
      irradiation. MATERIALS AND METHODS: Chromosome analyses were carried out on 
      peripheral lymphocytes from 73 radiation-exposed residents from settlements along 
      the Techa River (Southern Urals, Russia) located 7-148 km downstream from the 
      site of release of liquid radioactive waste from the plutonium production 
      facility Mayak. Thirty-nine unexposed persons from non-contaminated areas were 
      used as controls. Whole-chromosome painting probes for chromosomes 1, 4 and 12 
      were used simultaneously with a pancentromeric probe. RESULTS: A significantly 
      elevated mean translocation frequency compared with controls was found for the 
      total study group and for either of two subgroups of inhabitants residing in 
      villages of the upper regions of the Techa River (7-60 km) during 1950 to 1951, 
      or in villages of the lower regions (78-148 km) until the time of blood sampling. 
      Within the first subgroup, subjects born between 1937 and 1949 showed higher 
      translocation frequencies than those born between 1914 and 1936. Collective 
      biodosimetry estimates for the various groups were between 0.24 and 0.54 Gy. 
      Individual dose estimates for seven subjects with at least five translocations 
      ranged between 0.77 and 1.80 Gy and compared well with doses reconstructed on the 
      basis of 90Sr whole-body counts (WBC) and electronic paramagnetic resonance (EPR) 
      measurements. Individual translocation frequencies from 40 subjects with existing 
      WBC data and calculated cumulative red bone marrow doses below 0.6 Gy fall within 
      the 95% prediction limits of the calibration curve. CONCLUSIONS: FISH-based 
      translocation measurements can provide useful information for a retrospective 
      biodosimetric interpretation. However, with the analysed number of cells, 
      individual estimates required for a reliable evaluation of this highly variable 
      exposure situation can be obtained only for a minority of subjects. In addition, 
      influence of a temporal decline on the yield of stable translocations cannot be 
      fully excluded.
FAU - Bauchinger, M
AU  - Bauchinger M
AD  - GSF-National Research Center for Environment and Health, Institute of 
      Radiobiology, Neuherberg, Germany.
FAU - Salassidis, K
AU  - Salassidis K
FAU - Braselmann, H
AU  - Braselmann H
FAU - Vozilova, A
AU  - Vozilova A
FAU - Pressl, S
AU  - Pressl S
FAU - Stephan, G
AU  - Stephan G
FAU - Snigiryova, G
AU  - Snigiryova G
FAU - Kozheurov, V P
AU  - Kozheurov VP
FAU - Akleyev, A
AU  - Akleyev A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Cesium Radioisotopes)
RN  - 0 (Radioactive Waste)
RN  - 0 (Strontium Radioisotopes)
RN  - 53023GN24M (Plutonium)
SB  - IM
MH  - Body Burden
MH  - Cells, Cultured
MH  - *Cesium Radioisotopes
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 4
MH  - Gamma Rays
MH  - Geography
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lymphocytes/cytology/*radiation effects
MH  - *Nuclear Reactors
MH  - Plutonium
MH  - Radiation Dosage
MH  - Radiation Monitoring/*methods
MH  - *Radioactive Waste
MH  - Retrospective Studies
MH  - Rural Population
MH  - Russia
MH  - *Strontium Radioisotopes
MH  - Time Factors
MH  - *Translocation, Genetic
EDAT- 1998/08/05 00:00
MHDA- 1998/08/05 00:01
CRDT- 1998/08/05 00:00
PHST- 1998/08/05 00:00 [pubmed]
PHST- 1998/08/05 00:01 [medline]
PHST- 1998/08/05 00:00 [entrez]
AID - 10.1080/095530098141852 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1998 Jun;73(6):605-12. doi: 10.1080/095530098141852.