PMID- 9691171
OWN - NLM
STAT- MEDLINE
DCOM- 19981019
LR  - 20180213
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 81
IP  - 1
DP  - 1998
TI  - Asynchronous replication of homologous alpha-satellite DNA loci in man is 
      associated with nondisjunction.
PG  - 26-35
AB  - We tested the hypothesis that loss of replication control of DNA loci associated 
      with human centromeres affects the main centromere function, namely, ensuring 
      proper sister chromatid separation and accurate chromosomal segregation during 
      cell division. Applying one-color fluorescence in situ hybridization (FISH) to 
      interphase nuclei, we studied the replication patterns of homologous DNA loci 
      associated with human centromeres (alpha-satellite sequences) of chromosome pairs 
      10, 11, 17, and X in PHA-stimulated lymphocytes of female cancer patients with a 
      familial predisposition to malignancy and normal, healthy women. Concomitantly, 
      we measured the rates of aneuploidy for these chromosomes in the same cells. To 
      elucidate the replication patterns of the various centromeric loci, we analyzed 
      the replication-dependent configuration signals obtained following FISH with four 
      chromosome-specific alpha-satellite probes. Our data showed an association 
      between replication timing of alpha-satellite sequences and centromeric function. 
      Chromosome pairs whose homologous alpha-satellite loci replicated highly 
      synchronously revealed low rates of aneuploidy, whereas chromosome pairs with a 
      slightly asynchronous replication pattern (i.e., short intervals between early- 
      and late-replicating loci) revealed intermediate rates of aneuploidy, and 
      chromosome pairs exhibiting asynchrony with long-time intervals between early- 
      and late-replicating loci showed the highest rate of aneuploidy.
FAU - Litmanovitch, T
AU  - Litmanovitch T
AD  - Department of Human Genetics, Sackler School of Medicine, Tel-Aviv University, 
      Tel-Aviv, (Israel).
FAU - Altaras, M M
AU  - Altaras MM
FAU - Dotan, A
AU  - Dotan A
FAU - Avivi, L
AU  - Avivi L
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Aneuploidy
MH  - Chromosomes, Human, Pair 10/genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - DNA Probes/genetics
MH  - DNA Replication/*genetics
MH  - DNA, Satellite/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Nondisjunction, Genetic
MH  - Ovarian Neoplasms/genetics
MH  - Time Factors
MH  - X Chromosome/genetics
EDAT- 1998/08/06 02:01
MHDA- 2000/08/16 11:00
CRDT- 1998/08/06 02:01
PHST- 1998/08/06 02:01 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1998/08/06 02:01 [entrez]
AID - ccg81026 [pii]
AID - 10.1159/000015003 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1998;81(1):26-35. doi: 10.1159/000015003.