PMID- 9691204
OWN - NLM
STAT- MEDLINE
DCOM- 19980908
LR  - 20180213
IS  - 0254-9670 (Print)
IS  - 0254-9670 (Linking)
VI  - 15
IP  - 2
DP  - 1998
TI  - In vivo and in vitro modulation of immune stimulatory capacity of primary 
      dendritic cells by adenovirus-mediated gene transduction.
PG  - 100-11
AB  - Dendritic cells (DCs) are potent antigen-presenting cells which are key 
      leukocytes in the initiation of cell-mediated organ graft rejection, antiviral 
      immunity, and antitumor responses. In this study we demonstrate that genetic 
      modification of primary human and mouse DCs by adenoviral gene transfer is an 
      effective means of induction and modulation of antigen presentation by DCs. An 
      adenovirus vector (AdLacZ) was used to express an intracellular model antigen 
      beta-galactosidase (beta-gal) in DCs. Our results show that 30-40% of precursor 
      dendritic cells (PDCs) derived from human umbilical cord blood and circulating 
      mature blood DCs express high levels beta-galactosidase (beta-gal) after 
      infection with AdLacZ with no cytopathic effect observed. In vitro, AdLacZ 
      transduced PDCs and DCs demonstrated a 10- to 20-fold higher mixed lymphocyte 
      reaction (MLR) stimulatory capacity as compared to that of monocytes. In vivo, 
      immunization with AdLacZ transduced mouse DCs resulted in more potent cytotoxic T 
      lymphocyte (CTL) responses against the predicted H-2 restricted beta-gal epitope 
      as compared to CTL responses obtained by beta-gal peptide pulsed DCs. Modulations 
      of the MLR stimulatory capacity of DCs were examined by expression of mouse B7 
      and CTLA-4Ig. The results show that expression of mouse B7 by a recombinant 
      adenoviral vector (Ad7) significantly enhances the MLR stimulatory capacity of 
      human DCs. In contrast, expression of CTLA-4Ig (AdCTLA-4Ig) reduces the MLR 
      stimulatory capacity of the transduced cells. We conclude that recombinant 
      adenovirus can readily be used for genetic modulation DC-induced immune responses 
      in vivo and in vitro. DCs targeted for induction of specific antigen responses or 
      for modulation of the immune stimulatory capacity may have a potential use in the 
      control of transplantation rejection or viral infections.
FAU - Sonderbye, L
AU  - Sonderbye L
AD  - Division of Nephrology, Milton S. Hershey Medical Center, Penn State University, 
      Hershey, PA 17033, USA.
FAU - Feng, S
AU  - Feng S
FAU - Yacoubian, S
AU  - Yacoubian S
FAU - Buehler, H
AU  - Buehler H
FAU - Ahsan, N
AU  - Ahsan N
FAU - Mulligan, R
AU  - Mulligan R
FAU - Langhoff, E
AU  - Langhoff E
LA  - eng
GR  - AI28734/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Exp Clin Immunogenet
JT  - Experimental and clinical immunogenetics
JID - 8411714
RN  - 0 (DNA Primers)
SB  - IM
MH  - Adenoviridae/*genetics
MH  - Animals
MH  - Antigen Presentation/genetics
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - Dendritic Cells/*immunology
MH  - Gene Expression
MH  - Gene Transfer Techniques
MH  - Genetic Engineering
MH  - Genetic Vectors
MH  - Humans
MH  - In Vitro Techniques
MH  - Lac Operon
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - *Transduction, Genetic
EDAT- 1998/08/06 02:01
MHDA- 2000/08/16 11:00
CRDT- 1998/08/06 02:01
PHST- 1998/08/06 02:01 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1998/08/06 02:01 [entrez]
AID - eci15100 [pii]
AID - 10.1159/000019060 [doi]
PST - ppublish
SO  - Exp Clin Immunogenet. 1998;15(2):100-11. doi: 10.1159/000019060.