PMID- 9696155
OWN - NLM
STAT- MEDLINE
DCOM- 19981008
LR  - 20191102
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 34
IP  - 3
DP  - 1998 Jun 15
TI  - Double-target fluorescence in situ hybridization distinguishes multiple 
      genetically aberrant clones in head and neck squamous cell carcinoma.
PG  - 113-20
AB  - Genomic heterogeneity has been observed in several solid tumor types. To 
      investigate this phenomenon in head and neck squamous cell carcinoma (HNSCC), we 
      analyzed macroscopically distinct tissue samples of 12 resected tumors by a 
      combination of fluorescence in situ hybridization (FISH) and DNA flow cytometry. 
      Using a panel of centromeric DNA probes, numerical chromosomal aberrations were 
      detected in 10 tumors, 9 of which showed a single DNA aneuploid peak. Imbalances 
      in chromosomal copy numbers resulted in unique patterns of chromosomal 
      aberrations for each tumor case. Two types of tumors could be distinguished, 
      i.e., tumors (n = 5) containing a single aneusomic clone and tumors (n = 5) with 
      multiple aneusomic clones. The center of this latter group of tumors was shown to 
      be genetically more heterogeneous than the tumor margin. In conclusion, this 
      study showed that 1) the pattern of chromosomal aberrations varies greatly 
      between different HNSCC, 2) a major clone with a specific pattern of chromosomal 
      aberrations has spread throughout most HNSCC, and 3) a subgroup of HNSCCs 
      contains additional clones with a different pattern of chromosomal aberrations. 
      Based on these results, HNSCC can be divided into a genetically more homogeneous 
      and a genetically more heterogeneous group.
FAU - Veltman, J A
AU  - Veltman JA
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital 
      Maastricht and University of Maastricht, The Netherlands. 
      j.veltman@molcelb.unimaas.nl
FAU - Hopman, A H
AU  - Hopman AH
FAU - van der Vlies, S A
AU  - van der Vlies SA
FAU - Bot, F J
AU  - Bot FJ
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - Manni, J J
AU  - Manni JJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/classification/*genetics/pathology
MH  - Chromosome Aberrations/*genetics
MH  - Clone Cells/classification/pathology
MH  - DNA Probes
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Flow Cytometry/methods
MH  - Genetic Heterogeneity
MH  - Head and Neck Neoplasms/classification/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
EDAT- 1998/08/08 04:43
MHDA- 2000/06/20 09:00
CRDT- 1998/08/08 04:43
PHST- 1998/08/08 04:43 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/08/08 04:43 [entrez]
AID - 10.1002/(SICI)1097-0320(19980615)34:3<113::AID-CYTO1>3.0.CO;2-I [pii]
AID - 10.1002/(sici)1097-0320(19980615)34:3<113::aid-cyto1>3.0.co;2-i [doi]
PST - ppublish
SO  - Cytometry. 1998 Jun 15;34(3):113-20. doi: 
      10.1002/(sici)1097-0320(19980615)34:3<113::aid-cyto1>3.0.co;2-i.