PMID- 9697873
OWN - NLM
STAT- MEDLINE
DCOM- 19980819
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 12
IP  - 8
DP  - 1998 Aug
TI  - Hepatocyte growth factor/scatter factor (HGF/SF) affects proliferation and 
      migration of myeloid leukemic cells.
PG  - 1195-203
AB  - Hepatocyte growth factor (HGF), also known as scatter factor (SF), is produced by 
      mesenchymal cells, including bone marrow (BM) stromal cells, and has mitogenic 
      and motogenic effects on a variety of cell types. Recently, a role has been 
      assigned to HGF/SF and its receptor, c-MET, in both normal and malignant 
      hemopoiesis. We investigated the function of HGF/SF on hemopoietic mononuclear 
      cells (MNC) from patients with acute myeloid leukemia (AML) and myelodysplastic 
      syndrome (MDS) with circulating blasts. In contrast to results with normal MNC, 
      HGF/SF alone stimulated the proliferation and colony formation of MNC from these 
      patients. MNC from some (4/13) of the AML patients also produced HGF/SF (0.1-0.2 
      ng/ml/day), while we could not detect HGF/SF in cultures from normal MNC. 
      Furthermore, it appeared that HGF/SF induced migration of leukemic cells in 
      Boyden using KG1a cells as a model for leukemic blasts. The membranes dividing 
      the two compartments of the Boyden chambers were coated with fibronectin. HGF/SF 
      significantly promoted migration in 3/5 samples of MDS patients and in 5/7 
      samples of AML patients. Supernatant of human BM stromal cells, which is 
      chemoattractive for normal human hemopoietic progenitor cells, also promoted 
      migration of MNC from 4/5 MDS patients and 6/7 AML patients. Since HGF/SF is one 
      of the growth factors produced by BM stromal cells, a neutralizing antibody 
      directed against HGF/SF was added to the BM stroma supernatant, which reduced 
      migration significantly in 2/3 MDS and in 3/6 AML responders to BM stroma 
      supernatant. In conclusion, HGF/SF promotes proliferation and migration of 
      hemopoietic cells from AML and MDS patients in vitro and may therefore contribute 
      to the malignant potential of these cells.
FAU - Weimar, I S
AU  - Weimar IS
AD  - Division of Immunology, Netherlands Cancer Institute, Amsterdam.
FAU - Voermans, C
AU  - Voermans C
FAU - Bourhis, J H
AU  - Bourhis JH
FAU - Miranda, N
AU  - Miranda N
FAU - van den Berk, P C
AU  - van den Berk PC
FAU - Nakamura, T
AU  - Nakamura T
FAU - de Gast, G C
AU  - de Gast GC
FAU - Gerritsen, W R
AU  - Gerritsen WR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Antigens, CD34)
RN  - 0 (Fibronectins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Acute Disease
MH  - Antigens, CD34/metabolism
MH  - Bone Marrow Cells/metabolism
MH  - Cell Adhesion
MH  - Cell Division
MH  - Cell Movement
MH  - Colony-Forming Units Assay
MH  - Fibronectins/metabolism
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Leukemia, Myeloid/*metabolism/pathology
MH  - Myelodysplastic Syndromes/metabolism/pathology
MH  - Stromal Cells/metabolism
EDAT- 1998/08/11 00:00
MHDA- 1998/08/11 00:01
CRDT- 1998/08/11 00:00
PHST- 1998/08/11 00:00 [pubmed]
PHST- 1998/08/11 00:01 [medline]
PHST- 1998/08/11 00:00 [entrez]
AID - 10.1038/sj.leu.2401080 [doi]
PST - ppublish
SO  - Leukemia. 1998 Aug;12(8):1195-203. doi: 10.1038/sj.leu.2401080.