PMID- 9698103
OWN - NLM
STAT- MEDLINE
DCOM- 19981112
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 62
IP  - 2
DP  - 1998 Jun
TI  - Detection of oxidative DNA damage by a monoclonal antibody: role of lysyl 
      residues in antigen binding.
PG  - 87-92
AB  - Hydroxyl radical, a prominent entity of reactive oxygen species, is known to 
      modify cellular DNA and has been implicated in several human diseases. A 
      previously described monoclonal antibody (Mab) against reactive oxygen 
      species-modified DNA (ROS-DNA), which preferentially recognizes ROS-modified 
      epitopes on DNA, was used in this study. The epsilon-amino groups of lysine of 
      the Mab were modified to study the role of these residues in Mab binding to 
      ROS-DNA. The results demonstrate that modification of lysyl residues paralleled 
      loss in Mab binding to ROS-DNA to the extent of 73%, suggesting the probable role 
      of these positively charged amino acid residues in the complementarily 
      determining regions of the Mab. The Mab was also used as an immunochemical probe 
      to detect oxidative DNA damage in vivo in SLE. The Mab distinctly recognized five 
      DNA samples out of eight from SLE patients and gave maximum inhibitions of 57, 
      58, 63, 64 and 70% in inhibition assay, while not reacting with DNA from normal, 
      healthy population which served as negative control. High recognition of DNA 
      isolates from SLE patients by the Mab having preferential binding to ROS-modified 
      epitopes indicates increased oxidative stress in these patients leading to DNA 
      damage which may contribute to the induction of antibodies cross-reacting with 
      native DNA (nDNA).
FAU - Ahmad, J
AU  - Ahmad J
AD  - Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, 
      India.
FAU - Ashok, B T
AU  - Ashok BT
FAU - Ali, R
AU  - Ali R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 9007-49-2 (DNA)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antigen-Antibody Reactions
MH  - Antigens/immunology
MH  - Cattle
MH  - DNA/*immunology
MH  - DNA Damage/*immunology
MH  - Humans
MH  - Lupus Erythematosus, Systemic/*genetics/immunology
MH  - Lysine/*immunology
MH  - *Oxidative Stress
EDAT- 1998/08/11 00:00
MHDA- 1998/08/11 00:01
CRDT- 1998/08/11 00:00
PHST- 1998/08/11 00:00 [pubmed]
PHST- 1998/08/11 00:01 [medline]
PHST- 1998/08/11 00:00 [entrez]
AID - S0165-2478(98)00024-8 [pii]
AID - 10.1016/s0165-2478(98)00024-8 [doi]
PST - ppublish
SO  - Immunol Lett. 1998 Jun;62(2):87-92. doi: 10.1016/s0165-2478(98)00024-8.