PMID- 9698673
OWN - NLM
STAT- MEDLINE
DCOM- 19980910
LR  - 20190719
IS  - 0007-1331 (Print)
IS  - 0007-1331 (Linking)
VI  - 82
IP  - 1
DP  - 1998 Jul
TI  - Urinary levels of monocyte chemo-attractant protein-1 correlate with tumour stage 
      and grade in patients with bladder cancer.
PG  - 118-21
AB  - OBJECTIVE: To determine if the chemokine monocyte chemo-attractant protein-1 
      (MCP-1) is produced locally in patients with bladder cancer and to analyse a 
      possible correlation between tumour stage, grade and metastatic spread, and the 
      urinary and systemic levels of MCP-1. PATIENTS SUBJECTS AND METHODS: Urine and 
      serum samples were obtained from 60 patients with bladder cancer and 20 control 
      subjects. Tumour stage, grade, metastasis and nodal status were assessed. MCP-1 
      levels in serum and urine were determined using a sandwich enzyme-linked 
      immunosorbent assay. Two transitional cell cancer cell lines (grade I and grade 
      III) were analysed for MCP-1 production under normal and nutritive-stress cell 
      culture. RESULTS: The correlation of urinary MCP-1 levels with tumour stage, 
      grade and distant metastasis was highly significant. Patients with stage T2-T4 
      bladder cancer had three to fourfold higher mean MCP-1 concentrations (pg/mL) in 
      their urine than those with T1 stage tumours or than the controls (controls 260; 
      T1 359; T2 967; T3 917; T4 1829; P < 0.005). A tumour grade of > GI and the 
      existence of distant metastasis (M1) also correlated significantly with higher 
      urinary MCP-1 levels (GI 373; GII 661; GIII 1111; M0 644; M1 1379; P < 0.05). No 
      differences in circulating serum MCP-1 level were detected between controls and 
      patients. The low-grade (GI) RT4 bladder cancer cell line produced only traces of 
      MCP-1, which did not change under nutritional stress; in contrast, the highly 
      malignant T24 bladder cancer cell line (GIII) spontaneously secreted large 
      amounts of MCP-1 (approximately 7000 pg/mL) which increased under nutritive 
      stress to 13,000 pg/mL. CONCLUSION: MCP-1, as a potent monocyte chemo-attractant 
      to tumour sites, is probably produced by bladder cancer cells; MCP-1 levels in 
      the vicinity of the tumour (i.e. urine) correlate significantly with TNM stage 
      and grade. As has already been shown in other neoplasms, the resulting 
      monocyte/macrophage infiltrate possibly facilitates tumour neovascularization and 
      tissue invasion. Therefore, MCP-1 levels in the urine of patients with bladder 
      cancer may be a prognostic marker for the natural course of the disease, and 
      modulation of this chemokine might be a future therapeutic approach for adjuvant 
      treatment of bladder cancer.
FAU - Amann, B
AU  - Amann B
AD  - Medical Department North, Community Hospital of Dortmund, Germany.
FAU - Perabo, F G
AU  - Perabo FG
FAU - Wirger, A
AU  - Wirger A
FAU - Hugenschmidt, H
AU  - Hugenschmidt H
FAU - Schultze-Seemann, W
AU  - Schultze-Seemann W
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Urol
JT  - British journal of urology
JID - 15740090R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Aged
MH  - Carcinoma, Transitional Cell/pathology/*urine
MH  - Chemokine CCL2/*urine
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Lymphatic Metastasis
MH  - Male
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/*urine
MH  - Neoplasm Staging
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder Neoplasms/pathology/*urine
EDAT- 1998/08/12 00:00
MHDA- 1998/08/12 00:01
CRDT- 1998/08/12 00:00
PHST- 1998/08/12 00:00 [pubmed]
PHST- 1998/08/12 00:01 [medline]
PHST- 1998/08/12 00:00 [entrez]
AID - 10.1046/j.1464-410x.1998.00675.x [doi]
PST - ppublish
SO  - Br J Urol. 1998 Jul;82(1):118-21. doi: 10.1046/j.1464-410x.1998.00675.x.