PMID- 9699273
OWN - NLM
STAT- MEDLINE
DCOM- 19980924
LR  - 20190915
IS  - 0268-9146 (Print)
IS  - 0268-9146 (Linking)
VI  - 29
IP  - 2
DP  - 1998 Apr
TI  - Physical assignment of six type I anchor loci to bovine chromosome 19 by 
      fluorescence in situ hybridization.
PG  - 130-4
AB  - A bovine bacterial artificial chromosome (BAC) library was screened for the 
      presence of eight type I anchor loci previously used within hybrid somatic cells 
      and an interspecies hybrid backcross to construct a genome map of bovine 
      chromosome 19 (BTA19). Six out of eight loci were identified in the BAC library 
      (NF1, CRYB1, CHRNB1, TP53, GH1 and P4HB). The BACs were then used in 
      single-colour fluorescence in situ hybridization (FISH) to assign these genes to 
      BTA19 band locations. Gene order was determined by single-colour FISH, and was 
      confirmed by dual-colour FISH to mitotic and meiotic chromosomes. The order, 
      centromere-NF1-CRYB1-CHRNB1-TP53-GH1-P4HB, was in agreement with the order 
      determined by linkage analyses. In addition, the order of CHRNB1 and TP53, 
      previously unresolved by linkage analyses, was established. These data provide 
      high-resolution cytogenetic anchorage of the BTA19 genome map from chromosome 
      bands 14-22.
FAU - Gallagher, D S Jr
AU  - Gallagher DS Jr
AD  - Department of Animal Science, Texas A&M University, College Station 77843, USA.
FAU - Yang, Y P
AU  - Yang YP
FAU - Burzlaff, J D
AU  - Burzlaff JD
FAU - Womack, J E
AU  - Womack JE
FAU - Stelly, D M
AU  - Stelly DM
FAU - Davis, S K
AU  - Davis SK
FAU - Taylor, J F
AU  - Taylor JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
RN  - 36015-30-2 (Propidium)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
RN  - LHQ7J5KV9B (Bisbenzimidazole)
SB  - IM
MH  - Animals
MH  - Bisbenzimidazole/chemistry
MH  - Cattle/*genetics
MH  - Chromosome Mapping/*veterinary
MH  - Fluorescein-5-isothiocyanate/chemistry
MH  - Fluorescent Dyes/chemistry
MH  - *Genetic Markers
MH  - In Situ Hybridization, Fluorescence/*veterinary
MH  - Polymerase Chain Reaction/veterinary
MH  - Polymorphism, Restriction Fragment Length
MH  - Propidium/chemistry
EDAT- 1998/08/12 05:15
MHDA- 2001/03/28 10:01
CRDT- 1998/08/12 05:15
PHST- 1998/08/12 05:15 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/08/12 05:15 [entrez]
AID - 10.1046/j.1365-2052.1998.00239.x [doi]
PST - ppublish
SO  - Anim Genet. 1998 Apr;29(2):130-4. doi: 10.1046/j.1365-2052.1998.00239.x.