PMID- 9700494
OWN - NLM
STAT- MEDLINE
DCOM- 19981030
LR  - 20231213
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 24
IP  - 1
DP  - 1998 Sep
TI  - Dye coupling between spinal cord oligodendrocytes: differences in coupling 
      efficiency between gray and white matter.
PG  - 108-20
AB  - Oligodendrocytes express two gap junction proteins, connexin32 (Cx32) and Cx45. 
      To test for functional coupling between oligodendrocytes, cells were filled with 
      the (Cx32-permeable) dyes Lucifer Yellow (LY) and Neurobiotin. Cells in slices 
      from rat spinal cord were dialyzed via the patch pipette containing the dye while 
      recording with the patch-clamp technique. The dye-labeled cells were identified 
      as oligodendrocytes by their characteristic pattern of membrane currents and by 
      morphology. In gray matter, 18% of the injected cells (N = 94) were coupled to 
      more than three adjacent cells (slices from postnatal day 1 to 19). In contrast, 
      in white matter, the dye was restricted to the injected cell (N = 63 for Lucifer 
      Yellow injection only; N = 11 for LY and Neurobiotin) indicating a lack of 
      functional coupling. Immunolabeling of Cx32 in mature oligodendrocytes of white 
      matter revealed that the gap junction protein is localized on the cell bodies and 
      abaxonal processes which occupy non-overlapping territories. In immature white 
      matter and gray matter, Cx32 is mostly concentrated in the somatic region of the 
      cells. In addition to Cx32, we have obtained immunocytochemical data that 
      oligodendrocytes can express Cx45 with a labeling pattern different from the Cx32 
      expression. Two alternative interpretations of the coupling data are discussed: 
      1) that the presence of Cx32 in mature white matter oligodendrocytes does not 
      serve for communication between cells, but rather for communication within 
      oligodendrocytes in the sense of autocellular coupling, or 2) that the glial 
      syncytium is furnished with a high degree of functional rectification at the 
      oligodendrocytic side.
FAU - Pastor, A
AU  - Pastor A
AD  - Max Delbruck Center for Molecular Medicine, Berlin-Buch, Germany.
FAU - Kremer, M
AU  - Kremer M
FAU - Moller, T
AU  - Moller T
FAU - Kettenmann, H
AU  - Kettenmann H
FAU - Dermietzel, R
AU  - Dermietzel R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Connexins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Isoquinolines)
RN  - 0 (connexin 45)
RN  - 0 (neurobiotin)
RN  - 6SO6U10H04 (Biotin)
RN  - 9654F8OVKE (lucifer yellow)
SB  - IM
MH  - Animals
MH  - Biotin/*analogs & derivatives
MH  - Connexins/metabolism
MH  - Electrophysiology
MH  - *Fluorescent Dyes
MH  - In Vitro Techniques
MH  - *Isoquinolines
MH  - Oligodendroglia/*physiology
MH  - Periaqueductal Gray/cytology/physiology
MH  - Rats
MH  - Spinal Cord/*cytology/metabolism
MH  - Staining and Labeling
MH  - Tissue Distribution
MH  - Gap Junction beta-1 Protein
EDAT- 1998/08/13 00:00
MHDA- 1998/08/13 00:01
CRDT- 1998/08/13 00:00
PHST- 1998/08/13 00:00 [pubmed]
PHST- 1998/08/13 00:01 [medline]
PHST- 1998/08/13 00:00 [entrez]
AID - 10.1002/(SICI)1098-1136(199809)24:1<108::AID-GLIA11>3.0.CO;2-V [pii]
PST - ppublish
SO  - Glia. 1998 Sep;24(1):108-20.