PMID- 9701402
OWN - NLM
STAT- MEDLINE
DCOM- 19990519
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 32
IP  - 4
DP  - 1998 Aug 1
TI  - Automatic detection of stable and unstable chromosome aberrations visualized by 
      three-color imaging after fluorescence in situ hybridization with a painting and 
      a pancentromeric DNA probe.
PG  - 327-36
AB  - Two-color fluorescence in situ hybridization (FISH) in combination with digital 
      image analysis was used to develop an automatic system for the detection and 
      classification of chromosome aberrations. Algorithms were developed for the 
      automatic thresholding of the three digitized images: an FITC image representing 
      specific painted chromosomes, a TRITC image representing the centromeres of all 
      chromosomes, and a DAPI image representing all the counterstained chromosomes. A 
      further algorithm was developed for the automatic classification of the different 
      types of chromosome aberrations, such as translocations, dicentrics, and 
      fragments. For this study, a dataset of 252 metaphases were digitized and 
      analyzed automatically as well as manually. Of these metaphases, 81.3% could be 
      correctly classified by the algorithm. The error rate was reduced to 9.3% by 
      automatically excluding the detected clusters and artifacts. The average analysis 
      time per metaphase was 34.5 s without any user intervention.
FAU - Coco-Martin, J M
AU  - Coco-Martin JM
AD  - Division of Experimental Therapy, The Netherlands Cancer Institute, Amsterdam.
FAU - Lolkus, M
AU  - Lolkus M
FAU - Ottenheim, C P
AU  - Ottenheim CP
FAU - Oomen, L C
AU  - Oomen LC
FAU - Blommestijn, G J
AU  - Blommestijn GJ
FAU - Begg, A C
AU  - Begg AC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adenocarcinoma
MH  - Algorithms
MH  - Chromosome Aberrations/*genetics
MH  - *DNA Probes
MH  - Decision Trees
MH  - Diagnostic Imaging/*methods
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms
MH  - Metaphase
MH  - Trinucleotide Repeat Expansion/*genetics/radiation effects
MH  - Tumor Cells, Cultured
EDAT- 1998/08/13 02:04
MHDA- 2000/06/20 09:00
CRDT- 1998/08/13 02:04
PHST- 1998/08/13 02:04 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/08/13 02:04 [entrez]
AID - 10.1002/(SICI)1097-0320(19980801)32:4<327::AID-CYTO10>3.0.CO;2-O [pii]
PST - ppublish
SO  - Cytometry. 1998 Aug 1;32(4):327-36.