PMID- 9701450
OWN - NLM
STAT- MEDLINE
DCOM- 19981112
LR  - 20061115
IS  - 0094-6176 (Print)
IS  - 0094-6176 (Linking)
VI  - 24
IP  - 3
DP  - 1998
TI  - The influence of beta2-glycoprotein I on tissue factor activity.
PG  - 211-5
AB  - The beta2-glycoprotein I (beta2-GPI) is known for its procoagulant as well as 
      anticoagulant properties. The influence of beta2-GPI on tissue factor (TF) 
      activity was investigated by chromogenic assays for both factor Xa generation and 
      factor VIIa activity. When 1.36 mg/mL beta2-GPI was added to phospholipids prior 
      to the addition of TF and factor VIIa, the TF activity was moderately inhibited 
      by beta2-GPI in a concentration-dependent manner. Inhibition of TF activity by 
      beta2-GPI required incubation for at least 20 min. When beta2-GPI was added to 
      the mixture of phospholipids and TF followed by the addition of factor VIIa, the 
      TF activity was little affected by the addition of beta2-GPI. The TF activity was 
      inhibited when beta2-GPI was added after forming a complex with TF and factor 
      VIIa. The results suggest that beta2-GPI inhibits both the reconstitution of TF 
      with phospholipids and factor Xa generation by the TF factor-VIIa complex but 
      does not affect the formation of a TF factor-VIIa complex.
FAU - Ieko, M
AU  - Ieko M
AD  - Department of Internal Medicine, Health Science University of Hokkaido, 
      Ishikari-Toubetsu, Japan.
FAU - Yasukouchi, T
AU  - Yasukouchi T
FAU - Sawada, K I
AU  - Sawada KI
FAU - Koike, T
AU  - Koike T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Semin Thromb Hemost
JT  - Seminars in thrombosis and hemostasis
JID - 0431155
RN  - 0 (Anticoagulants)
RN  - 0 (Glycoproteins)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 3.4.21.21 (Factor VIIa)
RN  - EC 3.4.21.6 (Factor Xa)
SB  - IM
MH  - Animals
MH  - Anticoagulants/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Factor VIIa/drug effects/*metabolism
MH  - Factor Xa/drug effects/*metabolism
MH  - Glycoproteins/*pharmacology
MH  - Humans
MH  - Mice
MH  - Thromboplastin/*antagonists & inhibitors/metabolism
MH  - beta 2-Glycoprotein I
EDAT- 1998/08/13 00:00
MHDA- 1998/08/13 00:01
CRDT- 1998/08/13 00:00
PHST- 1998/08/13 00:00 [pubmed]
PHST- 1998/08/13 00:01 [medline]
PHST- 1998/08/13 00:00 [entrez]
AID - 10.1055/s-2007-995844 [doi]
PST - ppublish
SO  - Semin Thromb Hemost. 1998;24(3):211-5. doi: 10.1055/s-2007-995844.