PMID- 9701471
OWN - NLM
STAT- MEDLINE
DCOM- 19981118
LR  - 20211203
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 23
IP  - 2
DP  - 1998 Aug
TI  - Sequential expression of bone morphogenetic protein, tumor necrosis factor, and 
      their receptors in bone-forming reaction after mouse femoral marrow ablation.
PG  - 127-33
AB  - Tumor necrosis factor-alpha (TNF-alpha) is considered a promoter of bone 
      resorption and a suppressor of osteogenesis, whereas bone morphogenetic protein 
      (BMP) is a promoter of bone formation. In the present study, the osteogenic 
      potential of the medullary cavity after bone marrow ablation was evaluated in 
      association with the pattern of BMP, bone morphogenetic protein receptor (BMPR), 
      TNF-alpha, and tumor necrosis factor receptor (TNFR) expression. Immunostaining, 
      in situ hybridization, and TRAP staining were performed following marrow 
      ablation. By day 4, BMP-4 mRNA was detected by in situ hybridization in growing 
      undifferentiated cells and, on day 7, the osteoblastic cells that covered 
      abundant woven bone also showed evidence of BMP-4 expression. BMPR-IA and BMPR-II 
      were immunolocalized from days 4 to 10 after ablation, and became negative on 
      days 21 and 28. At 10 days postablation, osteoclasts were revealed by TRAP 
      staining. TNF-alpha expression disappeared transiently after ablation and then 
      reappeared on day 7, predominantly in osteoblastic cells. On days 7, 10, and 14, 
      immunostaining for TNFR-I was observed in osteoblasts lining the woven bone and 
      later disappeared. No evidence of TNFR-II staining was observed on osteoblastic 
      cells throughout the reaction. From day 14, newly formed bone decreased in 
      quantity and was replaced by hematopoietic cells and, by day 28, the bone marrow 
      had regenerated to its original state. This study suggests that TNF-alpha is 
      produced and secreted by the osteoblast and acts on these cells in an autocrine 
      manner to suppress osteoblastic function. TNF-alpha may also play a role in the 
      recruitment of osteoclasts because TRAP-positive osteoclasts appeared after 
      TNF-alpha expression.
FAU - Shimizu, T
AU  - Shimizu T
AD  - Department of Orthopaedic Surgery, School of Medicine, Shinshu University, 
      Matsumoto, Japan. tshimiz@med.shinshu-u.ac.jp
FAU - Mehdi, R
AU  - Mehdi R
FAU - Yoshimura, Y
AU  - Yoshimura Y
FAU - Yoshikawa, H
AU  - Yoshikawa H
FAU - Nomura, S
AU  - Nomura S
FAU - Miyazono, K
AU  - Miyazono K
FAU - Takaoka, K
AU  - Takaoka K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Bmp4 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.30 (Bmpr1a protein, mouse)
RN  - EC 2.7.11.30 (Bmpr2 protein, mouse)
RN  - EC 2.7.11.30 (Bone Morphogenetic Protein Receptors, Type I)
RN  - EC 2.7.11.30 (Bone Morphogenetic Protein Receptors, Type II)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Acp5 protein, mouse)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/analysis
MH  - Animals
MH  - Bone Density
MH  - Bone Marrow/*physiology/surgery
MH  - Bone Morphogenetic Protein 4
MH  - Bone Morphogenetic Protein Receptors, Type I
MH  - Bone Morphogenetic Protein Receptors, Type II
MH  - Bone Morphogenetic Proteins/*biosynthesis
MH  - Bone Remodeling
MH  - Cell Differentiation/physiology
MH  - Femur/diagnostic imaging/physiology/surgery
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Isoenzymes/analysis
MH  - Mice
MH  - Osteoblasts/cytology/metabolism
MH  - Protein Serine-Threonine Kinases/*biosynthesis
MH  - RNA, Messenger/biosynthesis
MH  - Radiography
MH  - Receptors, Growth Factor/*biosynthesis
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 1998/08/13 00:00
MHDA- 1998/08/13 00:01
CRDT- 1998/08/13 00:00
PHST- 1998/08/13 00:00 [pubmed]
PHST- 1998/08/13 00:01 [medline]
PHST- 1998/08/13 00:00 [entrez]
AID - S8756328298000866 [pii]
AID - 10.1016/s8756-3282(98)00086-6 [doi]
PST - ppublish
SO  - Bone. 1998 Aug;23(2):127-33. doi: 10.1016/s8756-3282(98)00086-6.