PMID- 9704567
OWN - NLM
STAT- MEDLINE
DCOM- 19980828
LR  - 20220716
IS  - 1355-008X (Print)
IS  - 1355-008X (Linking)
VI  - 8
IP  - 2
DP  - 1998 Apr
TI  - Tumor necrosis factor-alpha (TNF-alpha) inhibits steroidogenesis of bovine 
      ovarian granulosa and thecal cells in vitro. Involvement of TNF-alpha receptors.
PG  - 109-15
AB  - The effect of recombinant bovine tumor necrosis factor-alpha (TNF-alpha) on 
      steroidogenesis and numbers of bovine ovarian granulosa and thecal cells has been 
      studied, and specific binding sites for 125I-TNF-alpha on ovarian cells have been 
      determined. Granulosa cells have been examined from small (surface diameter 1-5 
      mm) follicles, whereas thecal cells from large (> or = 8 mm) follicles were 
      utilized. Increasing doses of TNF-alpha significantly attenuated insulin- and 
      IGF-I-induced estradiol production by granulosa cells from small follicles, but 
      had no effect on basal estradiol production. Moreover, TNF-alpha significantly 
      attenuated insulin- and LH-induced androstenedione production by thecal cells 
      from large follicles. TNF-alpha had little or no effect on the numbers of 
      granulosa and thecal cells in these same studies. Specific high-affinity, 
      low-capacity binding of 125I-TNF-alpha was also demonstrable in granulosa and 
      thecal cells. Thus, it appears that TNF-alpha inhibits insulin- and IGF-I-induced 
      estradiol production by granulosa cells and androstenedione production by thecal 
      cells via TNF-alpha binding to its own receptor.
FAU - Spicer, L J
AU  - Spicer LJ
AD  - Department of Animal Science, Oklahoma State University, Stillwater 74078, USA. 
      IGFLEO@okway.okstate.edu
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrine
JT  - Endocrine
JID - 9434444
RN  - 0 (Insulin)
RN  - 0 (Steroids)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 409J2J96VR (Androstenedione)
RN  - 4TI98Z838E (Estradiol)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Androstenedione/biosynthesis
MH  - Animals
MH  - Cattle/*metabolism
MH  - Cells, Cultured
MH  - Estradiol/biosynthesis
MH  - Female
MH  - Granulosa Cells/drug effects/*metabolism
MH  - Insulin/pharmacology
MH  - Insulin-Like Growth Factor I/pharmacology
MH  - Luteinizing Hormone/pharmacology
MH  - Steroids/*biosynthesis
MH  - Theca Cells/drug effects/*metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1998/08/15 00:00
MHDA- 1998/08/15 00:01
CRDT- 1998/08/15 00:00
PHST- 1997/09/24 00:00 [received]
PHST- 1997/11/10 00:00 [revised]
PHST- 1997/12/17 00:00 [accepted]
PHST- 1998/08/15 00:00 [pubmed]
PHST- 1998/08/15 00:01 [medline]
PHST- 1998/08/15 00:00 [entrez]
AID - ENDO:8:2:109 [pii]
AID - 10.1385/ENDO:8:2:109 [doi]
PST - ppublish
SO  - Endocrine. 1998 Apr;8(2):109-15. doi: 10.1385/ENDO:8:2:109.