PMID- 9705162
OWN - NLM
STAT- MEDLINE
DCOM- 19981106
LR  - 20190905
IS  - 0340-3696 (Print)
IS  - 0340-3696 (Linking)
VI  - 290
IP  - 6
DP  - 1998 Jun
TI  - Effects of cetirizine dihydrochloride on human lymphocytes in vitro: micronucleus 
      induction. Evaluation of clastogenic and aneugenic potential using CREST and FISH 
      assays.
PG  - 312-8
AB  - Cetirizine dihydrocloride, a widely administered antiallergic drug with the amine 
      piperazine in its molecule, was studied as to its ability to cause micronucleus 
      formation in human lymphocyte cultures treated in vitro. Peripheral lymphocytes 
      from four different donors were cultured and treated with different 
      concentrations of the compound. Cetirizine dihydrocloride was shown to induce 
      enhanced micronucleus frequency in a dose-dependent manner, although lymphocytes 
      from the different donors showed different susceptibilities to the compound. The 
      content of induced micronuclei was investigated in one of the four donors by two 
      independent assays, CREST (the application of antikinetochore antibodies) and 
      FISH (fluorescence in situ hybridization) on cytochalasin B-formed binucleated 
      cells. It was shown that the induced micronuclei resulted from breakage events as 
      well as chromosome loss, thus characterizing cetirizine dihydrocloride as both 
      clastogen and aneugen. Since our results were derived only from in vitro 
      experiments, we believe that an extensive in vivo study is necessary before 
      drawing conclusions as to the effects of cetirizine dihydrochloride in patients.
FAU - Vlastos, D
AU  - Vlastos D
AD  - Department of Biology, University of Patras, Greece.
FAU - Stephanou, G
AU  - Stephanou G
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arch Dermatol Res
JT  - Archives of dermatological research
JID - 8000462
RN  - 0 (Anti-Allergic Agents)
RN  - YO7261ME24 (Cetirizine)
SB  - IM
CIN - Arch Dermatol Res. 1999 Apr;291(4):244-5. PMID: 10335924
MH  - Adult
MH  - Aneuploidy
MH  - Anti-Allergic Agents/administration & dosage/*pharmacology
MH  - Cell Nucleus/drug effects/genetics/pathology
MH  - Centromere/drug effects/genetics
MH  - Cetirizine/administration & dosage/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetochores/drug effects/immunology
MH  - Lymphocytes/*drug effects/metabolism/pathology
MH  - Male
MH  - Micronuclei, Chromosome-Defective/drug effects/immunology/metabolism
MH  - Micronucleus Tests
MH  - Mutagenesis/drug effects/genetics
MH  - Regression Analysis
EDAT- 1998/08/15 00:00
MHDA- 1998/08/15 00:01
CRDT- 1998/08/15 00:00
PHST- 1998/08/15 00:00 [pubmed]
PHST- 1998/08/15 00:01 [medline]
PHST- 1998/08/15 00:00 [entrez]
AID - 10.1007/s004030050310 [doi]
PST - ppublish
SO  - Arch Dermatol Res. 1998 Jun;290(6):312-8. doi: 10.1007/s004030050310.