PMID- 9705171
OWN - NLM
STAT- MEDLINE
DCOM- 19981007
LR  - 20190831
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 73
IP  - 1
DP  - 1998 Jul
TI  - Human herpesvirus type 6 variants identified by single-strand conformation 
      polymorphism analysis.
PG  - 21-9
AB  - Six human herpesvirus 6 (HHV-6) variants were analyzed for heterogeneity using 
      the polymerase chain reaction (PCR) and single-strand conformation polymorphism 
      (SSCP). Two independent DNA regions were selected: a fragment of the gene U11 
      (position 18966-21578) coding for a basic phosphoprotein, the major antigenic 
      structural protein pp100; and a fragment from an open reading frame (ORF) area of 
      the gene U67, previously referred to as 13R (position 102458-103519), coding for 
      a product of unknown function. The two PCR systems based on the above DNA 
      sequences yielded products of 187 bp and 223 bp, respectively. DNA obtained from 
      three laboratory reference strains (U1102, R104 and St.W.) and from HHV-6 
      infected peripheral white blood cells of bone marrow transplant patients and 
      blood donors was used to test the applicability of two different SSCP analysis 
      systems for the identification of HHV-6 variants using amplicons derived by PCR 
      from the two genomic regions described above (U11 [pp100], U67 [13R-ORF]). The 
      generation of characteristic SSCP patterns enables the rapid differentiation of 
      HHV-6 A and B strains for the classification of variants derived from clinical 
      samples, reducing the need for expensive and time-consuming direct sequencing 
      analyses.
FAU - Wilborn, F
AU  - Wilborn F
AD  - Virchow-Klinikum, Abteilung fur Innere Medizin mit Schwerpunkt Hamatologie und 
      Onkologie, Humboldt Universitat, Berlin, Germany.
FAU - Binder, T
AU  - Binder T
FAU - Neipel, F
AU  - Neipel F
FAU - Blasczyk, R
AU  - Blasczyk R
FAU - Siegert, W
AU  - Siegert W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA, Viral)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Base Sequence
MH  - Blood Cells
MH  - Cell Line
MH  - DNA Restriction Enzymes
MH  - DNA, Viral/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Genetic Variation
MH  - Herpesvirus 6, Human/classification/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Point Mutation
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Single-Stranded Conformational
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 1998/08/15 00:00
MHDA- 1998/08/15 00:01
CRDT- 1998/08/15 00:00
PHST- 1998/08/15 00:00 [pubmed]
PHST- 1998/08/15 00:01 [medline]
PHST- 1998/08/15 00:00 [entrez]
AID - S0166093498000330 [pii]
AID - 10.1016/s0166-0934(98)00033-0 [doi]
PST - ppublish
SO  - J Virol Methods. 1998 Jul;73(1):21-9. doi: 10.1016/s0166-0934(98)00033-0.