PMID- 9705247
OWN - NLM
STAT- MEDLINE
DCOM- 19981106
LR  - 20181201
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 25
IP  - 1
DP  - 1998 Jul
TI  - Selective inhibition of TNF-alpha induced cell adhesion molecule gene expression 
      by tanapox virus.
PG  - 33-41
AB  - Poxviruses encode virulence factors that have been identified as proteins that 
      are secreted from infected host cells. Some of these secretory proteins impede 
      host immune defences. We have previously demonstrated that tanapox virus (TPV) 
      infected cells secrete an early 38 kDa glycopeptide that binds to human (h) 
      interferon-gamma, hIL-2, and hIL-5. We now show an additional activity in the 
      supernatant from TPV infected cells that down-regulates the expression of tumour 
      necrosis factor-alpha (TNF-alpha) induced cell adhesion molecule gene expression. 
      This activity was not detected in mock infected cells. Enzyme linked 
      immunosorbent assays (ELISA) on primary human endothelial cells, show the 
      induction of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and 
      intercellular adhesion molecule-1 (ICAM-1) following TNF-alpha or IL-1 beta 
      treatment, as expected. Supernatant from TPV infected cells significantly 
      decreased the TNF-alpha but not IL-1 beta-induced expression of these molecules. 
      Mobility shift assays and Northern blot analyses further show that the 
      supernatant from TPV infected cells inhibited TNF-alpha-induced activation of the 
      nuclear transcription factor-kappa B (NF-kappa B) and transcriptional activation 
      of the E-selectin, VCAM-1 and ICAM-1 genes. Based on TNF-alpha affinity 
      chromatography, this activity appears to be associated with a 38 kDa 
      glycopeptide.
FAU - Paulose, M
AU  - Paulose M
AD  - Department of Biological Sciences, Western Michigan University, Kalamazoo 49008, 
      USA.
FAU - Bennett, B L
AU  - Bennett BL
FAU - Manning, A M
AU  - Manning AM
FAU - Essani, K
AU  - Essani K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (E-Selectin)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Aotidae
MH  - Blotting, Northern
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Cell Line/metabolism/virology
MH  - Down-Regulation
MH  - E-Selectin/genetics/metabolism
MH  - Endothelium, Vascular
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - NF-kappa B/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/*physiology
MH  - Umbilical Veins
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
MH  - Virulence
MH  - Yatapoxvirus/immunology/*pathogenicity
EDAT- 1998/08/15 00:00
MHDA- 1998/08/15 00:01
CRDT- 1998/08/15 00:00
PHST- 1998/08/15 00:00 [pubmed]
PHST- 1998/08/15 00:01 [medline]
PHST- 1998/08/15 00:00 [entrez]
AID - S0882-4010(98)90213-3 [pii]
AID - 10.1006/mpat.1998.0213 [doi]
PST - ppublish
SO  - Microb Pathog. 1998 Jul;25(1):33-41. doi: 10.1006/mpat.1998.0213.