PMID- 9705262
OWN - NLM
STAT- MEDLINE
DCOM- 19980908
LR  - 20191210
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 248
IP  - 1
DP  - 1998 Aug 15
TI  - Control of baculovirus polyhedrin gene expression by very late factor 1.
PG  - 131-8
AB  - vlf-1 is a baculovirus gene that regulates very late gene expression (J. R. 
      McLachlin and L. K. Miller, J. Virol., 68, 7746-7756, 1994) and also plays a 
      crucial role in the replication of the budded form of Autographa californica 
      nuclear polyhedrosis virus (AcMNPV) (S. Yang and L. K. Miller, "Expression and 
      mutational analysis of the baculovirus very late factor 1 (vlf-1) gene." 
      Virology, 245, 99-109, 1998). To examine the influence of vlf-1 expression on 
      baculovirus infection, we constructed recombinant viruses that expressed only low 
      levels of VLF-1 and recombinants with vlf-1 under the control of different 
      promoters. Viruses with mutant alleles of vlf-1 that produced low levels of VLF-1 
      replicated the budded form of the virus normally but produced no occlusion 
      bodies. Thus, a higher concentration of VLF-1 was needed to activate very late 
      gene expression than was needed to support budded virus production. By altering 
      the level and/or timing of vlf-1 expression, the timing of polyhedrin gene (polh) 
      expression, which normally occurs very late in infection, could be advanced or 
      delayed. Early overexpression of vlf-1 increased the level of expression from the 
      polh promoter but caused premature cellular disintegration. The data indicate 
      that VLF-1 is the limiting factor in very late gene expression and that the level 
      of VLF-1 controls the onset of occlusion.
CI  - Copyright 1998 Academic Press.
FAU - Yang, S
AU  - Yang S
AD  - Department of Genetics, University of Georgia, Athens, Georgia, 30602, USA.
FAU - Miller, L K
AU  - Miller LK
LA  - eng
GR  - AI23719/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Ac-vlf-1 protein, Autographa californica nucleopolyhedrovirus)
RN  - 0 (Occlusion Body Matrix Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (polyhedrin protein, Nucleopolyhedrovirus)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Gene Expression Regulation, Viral
MH  - Genes, Viral
MH  - Nucleopolyhedroviruses/genetics/metabolism/*physiology
MH  - Occlusion Body Matrix Proteins
MH  - Promoter Regions, Genetic
MH  - Recombinant Proteins/biosynthesis
MH  - Spodoptera
MH  - Transcription Factors/*biosynthesis/physiology
MH  - Transfection
MH  - Viral Proteins/*biosynthesis/genetics/physiology
MH  - Viral Structural Proteins/genetics
MH  - Virus Replication
EDAT- 1998/08/15 00:00
MHDA- 1998/08/15 00:01
CRDT- 1998/08/15 00:00
PHST- 1998/08/15 00:00 [pubmed]
PHST- 1998/08/15 00:01 [medline]
PHST- 1998/08/15 00:00 [entrez]
AID - S0042-6822(98)99272-8 [pii]
AID - 10.1006/viro.1998.9272 [doi]
PST - ppublish
SO  - Virology. 1998 Aug 15;248(1):131-8. doi: 10.1006/viro.1998.9272.