PMID- 9705266
OWN - NLM
STAT- MEDLINE
DCOM- 19980908
LR  - 20041117
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 248
IP  - 1
DP  - 1998 Aug 15
TI  - Hepatitis C virus quasispecies in patients infected with HIV-1: correlation with 
      extrahepatic viral replication.
PG  - 164-71
AB  - Hepatitis C virus (HCV) sequences recovered from serum, peripheral blood 
      mononuclear cells (PBMCs), and various tissues from human immunodeficiency virus 
      type 1 (HIV-1) positive patients were compared by single strand conformational 
      polymorphism (SSCP) and sequencing. In five patients, paired serum and PBMCs 
      samples were analyzed while in two other patients multiple autopsy tissues were 
      studied. Sequences amplified from the NS5 and E2 regions were consistently 
      identical in the same patient; however, three PBMCs samples and three different 
      tissue samples (pancreas and adrenal gland in one patient and lymph node in the 
      other patient) contained 5' untranslated region (5'UTR) sequences that were 
      different from circulating sequences. The presence of 5' UTR sequences differing 
      from circulating sequences correlated with the presence of HCV RNA negative 
      strand, as the latter was detected by a Tth-based strand-specific assay in all 
      but one of these samples. These two independent lines of evidence: viral sequence 
      differences and the presence of RNA negative strand in the same tissues strongly 
      argue for the genuine presence of extrahepatic HCV replication, at least in the 
      setting of HIV-1 infection.
CI  - Copyright 1998 Academic Press.
FAU - Laskus, T
AU  - Laskus T
AD  - Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, 
      Pennsylvania, 15213, USA.
FAU - Radkowski, M
AU  - Radkowski M
FAU - Wang, L F
AU  - Wang LF
FAU - Jang, S J
AU  - Jang SJ
FAU - Vargas, H
AU  - Vargas H
FAU - Rakela, J
AU  - Rakela J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - HIV Seropositivity/blood/*complications/virology
MH  - *HIV-1
MH  - Hepacivirus/*classification/genetics/isolation & purification
MH  - Hepatitis C/blood/*complications/*virology
MH  - Humans
MH  - Leukocytes, Mononuclear/*virology
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - RNA, Viral/blood/*isolation & purification
MH  - Sequence Alignment
MH  - *Virus Replication
EDAT- 1998/08/15 00:00
MHDA- 1998/08/15 00:01
CRDT- 1998/08/15 00:00
PHST- 1998/08/15 00:00 [pubmed]
PHST- 1998/08/15 00:01 [medline]
PHST- 1998/08/15 00:00 [entrez]
AID - S0042-6822(98)99269-8 [pii]
AID - 10.1006/viro.1998.9269 [doi]
PST - ppublish
SO  - Virology. 1998 Aug 15;248(1):164-71. doi: 10.1006/viro.1998.9269.