PMID- 9707409
OWN - NLM
STAT- MEDLINE
DCOM- 19981009
LR  - 20220318
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 8
IP  - 16
DP  - 1998 Jul 30-Aug 13
TI  - The exchange factor Ras-GRF2 activates Ras-dependent and Rac-dependent 
      mitogen-activated protein kinase pathways.
PG  - 935-8
AB  - Ras and Rac are membrane-associated GTPases that function as molecular switches 
      activating intracellular mitogen-activated protein kinase (MAPK) cascades and 
      other effector pathways in response to extracellular signals [1]. Activation of 
      Ras and Rac into their GTP-bound conformations is directly controlled by specific 
      guanine-nucleotide exchange factors (GEFs), which catalyze GDP release. Several 
      Ras-specific GEFs that are related to the budding yeast protein Cdc25p have been 
      described, whereas GEFs for Rac-related GTPases contain a region that is 
      homologous to the oncoprotein DbI [2-3]. The Ras-GRF1 and Ras-GRF2 proteins, 
      which couple Ras activation to serpentine receptors and calcium signals, contain 
      both Cdc25 and DbI homology (DH) regions [3-4]. Here, we demonstrate that 
      Ras-GRF2 is a bifunctional signaling protein that is able to bind and activate 
      Ras and Rac, and thereby coordinate the activation of the 
      extracellular-signal-regulated kinase (ERK) and stress-activated protein kinase 
      (SAPK) pathways.
FAU - Fan, W T
AU  - Fan WT
AD  - Banting and Best Department of Medical Research Department of Molecular and 
      Medical Genetics University of Toronto Charles H. Best Institute 112 College 
      Street, Toronto, M5G 1L6, Canada.
FAU - Koch, C A
AU  - Koch CA
FAU - de Hoog, C L
AU  - de Hoog CL
FAU - Fam, N P
AU  - Fam NP
FAU - Moran, M F
AU  - Moran MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (ras Guanine Nucleotide Exchange Factors)
RN  - 0 (ras-GRF1)
RN  - 56092-81-0 (Ionomycin)
RN  - 6C74YM2NGI (Anisomycin)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Anisomycin/pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - Cell Cycle Proteins/metabolism
MH  - Cell Line
MH  - Enzyme Activation
MH  - GTP Phosphohydrolases/*metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - Guanine Nucleotide Exchange Factors
MH  - Humans
MH  - Ionomycin/pharmacology
MH  - Mitogen-Activated Protein Kinase 3
MH  - *Mitogen-Activated Protein Kinases
MH  - Phosphoprotein Phosphatases/metabolism
MH  - Proteins/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Signal Transduction
MH  - Transfection
MH  - rac GTP-Binding Proteins
MH  - ras Guanine Nucleotide Exchange Factors
MH  - ras Proteins/*metabolism
MH  - ras-GRF1
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - S0960-9822(07)00376-4 [pii]
AID - 10.1016/s0960-9822(07)00376-4 [doi]
PST - ppublish
SO  - Curr Biol. 1998 Jul 30-Aug 13;8(16):935-8. doi: 10.1016/s0960-9822(07)00376-4.