PMID- 9708707
OWN - NLM
STAT- MEDLINE
DCOM- 19980828
LR  - 20190826
IS  - 0886-4470 (Print)
IS  - 0886-4470 (Linking)
VI  - 124
IP  - 8
DP  - 1998 Aug
TI  - Malignancy detection by molecular cytogenetics in clinically normal mucosa 
      adjacent to head and neck tumors.
PG  - 847-51
AB  - OBJECTIVE: To identify the potential use of chromosome imbalances as biomarkers 
      for tumorigenesis in head and neck squamous cell carcinoma (HNSCC) by 
      fluorescence in situ hybridization (FISH). DESIGN: In this case-control study, 
      chromosome copy numbers were assessed in dual-target, dual-color FISH assays 
      using DNA probes specific for 14 human chromosomes (1, 2, 3, 6, 7, 8, 9, 10, 11, 
      12, 15, 17, X, and Y) applied to exfoliated epithelial cells. SETTING: University 
      medical center. PATIENTS: We examined 20 cell brushings (from 10 primary tumors 
      and 10 clinically normal margins) collected from 10 patients with HNSCC and 
      compared these with cell brushings from the oral cavity of 10 nonsmoker and 10 
      smoker control subjects. INTERVENTION: None. MAIN OUTCOME MEASURE: Chromosomal 
      aneuploidy. RESULTS: Specimens from nonsmokers displayed greater than 91% of 
      cells with normal signals, indicating high analytical sensitivity for the probes. 
      Specimens from smokers demonstrated large variability without significant 
      imbalance (P>.05) compared with those from nonsmokers. Tumor specimens from 
      patients with HNSCC displayed significant chromosomal imbalance (P<.05) for all 
      probes except chromosome Y. Similar imbalance, although in lower frequency, was 
      found in all clinically normal adjacent mucosa specimens. CONCLUSIONS: Interphase 
      FISH demonstrated great applicability in detecting chromosome imbalance 
      associated with malignancy in HNSCC and clinically normal adjacent cells, thereby 
      detecting subclinical tumorigenesis. A panel of chromosome probes (chromosomes 3, 
      8, 9, and 10) is proposed as an efficient and sensitive additional tool for 
      future routine screening of tumor margins and potential diagnosis of residual 
      disease in HNSCC.
FAU - Barrera, J E
AU  - Barrera JE
AD  - Department of Otolaryngology-Head and Neck Surgery, University of Colorado Health 
      Sciences Center, Denver 80262, USA. barreraj@york.uchsc.edu
FAU - Ai, H
AU  - Ai H
FAU - Pan, Z
AU  - Pan Z
FAU - Meyers, A D
AU  - Meyers AD
FAU - Varella-Garcia, M
AU  - Varella-Garcia M
LA  - eng
GR  - CA 46934/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Otolaryngol Head Neck Surg
JT  - Archives of otolaryngology--head & neck surgery
JID - 8603209
SB  - IM
CIN - Arch Otolaryngol Head Neck Surg. 1998 Aug;124(8):841-2. PMID: 9708706
MH  - Adult
MH  - Aged
MH  - *Aneuploidy
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Case-Control Studies
MH  - Female
MH  - Head and Neck Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mucous Membrane/pathology
MH  - Smoking/pathology
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - 10.1001/archotol.124.8.847 [doi]
PST - ppublish
SO  - Arch Otolaryngol Head Neck Surg. 1998 Aug;124(8):847-51. doi: 
      10.1001/archotol.124.8.847.