PMID- 9709414
OWN - NLM
STAT- MEDLINE
DCOM- 19980915
LR  - 20190512
IS  - 0008-6363 (Print)
IS  - 0008-6363 (Linking)
VI  - 38
IP  - 2
DP  - 1998 May
TI  - Release of chemoattractants for human monocytes from endothelial cells by 
      interaction with neutrophils.
PG  - 516-21
AB  - OBJECTIVE: The release of monocyte chemoattractant protein-1 (MCP-1) in the 
      vessel wall may lead to accumulation of monocytes in the subendothelial space. 
      The role of neutrophils (PMNL) in the initiation of this process is unknown. We 
      tested whether PMNL are able to induce the production and release of MCP-1 in 
      endothelial cells. METHODS: PMNL were allowed to interact with human umbilical 
      vein endothelial cell (HUVEC) monolayers in culture. Culture media were collected 
      and assessed for chemotactic activity on mononuclear leukocytes (MNC) or purified 
      monocytes in a modified Boyden chamber assay. Additionally, MCP-1 levels in 
      supernatants were quantified by ELISA. RESULTS: Media from unstimulated HUVEC 
      culture supernatants induced a slight increase (1.2-fold) of MNC and purified 
      monocyte chemotaxis, which was significantly augmented by addition of PMNL for 1 
      h (1.4-fold; P < 0.05). The increase in chemotaxis was time- and dose-dependent 
      and could be blocked by an anti-MCP-1 monoclonal antibody. Media obtained after 
      coculture of PMNL and HUVEC for 1-5 h contained increased amounts of MCP-1 as 
      measured by ELISA; addition of cycloheximide abolished this response. 
      CONCLUSIONS: Interaction of PMNL with endothelium induces the release of 
      functionally active MCP-1 suggesting that in the vascular wall, PMNL may play a 
      role in the recruitment of MNC.
FAU - Schratzberger, P
AU  - Schratzberger P
AD  - Department of Internal Medicine, Medical Faculty, University of Innsbruck, 
      Austria.
FAU - Dunzendorfer, S
AU  - Dunzendorfer S
FAU - Reinisch, N
AU  - Reinisch N
FAU - Kahler, C M
AU  - Kahler CM
FAU - Herold, M
AU  - Herold M
FAU - Wiedermann, C J
AU  - Wiedermann CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cardiovasc Res
JT  - Cardiovascular research
JID - 0077427
RN  - 0 (Chemokine CCL2)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Arteriosclerosis/*physiopathology
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Chemotaxis, Leukocyte
MH  - Coculture Techniques
MH  - Cycloheximide/pharmacology
MH  - Endothelium, Vascular/*metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear/drug effects
MH  - Neutrophils/*physiology
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Time Factors
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - S0008-6363(98)00014-5 [pii]
AID - 10.1016/s0008-6363(98)00014-5 [doi]
PST - ppublish
SO  - Cardiovasc Res. 1998 May;38(2):516-21. doi: 10.1016/s0008-6363(98)00014-5.