PMID- 9710121
OWN - NLM
STAT- MEDLINE
DCOM- 19980901
LR  - 20190706
IS  - 0009-7330 (Print)
IS  - 0009-7330 (Linking)
VI  - 83
IP  - 3
DP  - 1998 Aug 10
TI  - Importance of E-selectin for firm leukocyte adhesion in vivo.
PG  - 287-94
AB  - Leukocyte adhesion under flow is preferentially mediated by the selectins. In 
      this study we used intravital microscopy to investigate whether E-selectin may 
      promote firm leukocyte adhesion in vivo. E-Selectin is expressed by endothelial 
      cells activated with tumor necrosis factor-alpha (TNF-alpha) and causes slow 
      leukocyte rolling. Microinjection of formyl-methionyl-leucyl-phenylalanine (fMLP) 
      or macrophage inflammatory protein-2 (MIP-2) next to a venule of the 
      TNF-alpha-treated mouse cremaster muscle significantly increased the number of 
      adherent leukocytes. In gene-targeted mice homozygous for a null mutation in the 
      E-selectin gene or in wild-type mice treated with an E-selectin monoclonal 
      antibody (mAb), this response was significantly attenuated (by >80%). No such 
      defect was seen in intercellular adhesion molecule-1 (ICAM-1)-deficient mice. 
      E-Selectin-null mice showed more rapid leukocyte rolling than wild-type or 
      ICAM-1-deficient mice, resulting in significantly shortened leukocyte transit 
      times through venules. Topical application of fMLP onto the whole cremaster 
      muscle generated the same number of adherent leukocytes in wild-type and 
      E-selectin-deficient mice. We conclude that slow leukocyte rolling through 
      E-selectin results in long transit times, which are essential for efficient 
      leukocyte adhesion in response to a local chemotactic stimulus.
FAU - Ley, K
AU  - Ley K
AD  - Department of Biomedical Engineering, University of Virginia School of Medicine, 
      Charlottesville 22908, USA. kf13f@virginia.edu
FAU - Allietta, M
AU  - Allietta M
FAU - Bullard, D C
AU  - Bullard DC
FAU - Morgan, S
AU  - Morgan S
LA  - eng
GR  - R01 AI-32177/AI/NIAID NIH HHS/United States
GR  - R01 HL-54136/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (E-Selectin)
RN  - 0 (Monokines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Movement/drug effects
MH  - Chemokine CXCL2
MH  - Chemotactic Factors/administration & dosage/pharmacology
MH  - E-Selectin/*physiology
MH  - Hemodynamics/drug effects
MH  - Intercellular Adhesion Molecule-1/physiology
MH  - Leukocyte Count
MH  - Leukocytes/drug effects/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microinjections
MH  - Monokines/administration & dosage/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/administration & dosage/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - 10.1161/01.res.83.3.287 [doi]
PST - ppublish
SO  - Circ Res. 1998 Aug 10;83(3):287-94. doi: 10.1161/01.res.83.3.287.