PMID- 9711029
OWN - NLM
STAT- MEDLINE
DCOM- 19981021
LR  - 20061115
IS  - 0022-7722 (Print)
IS  - 0022-7722 (Linking)
VI  - 73
IP  - 3
DP  - 1998 Jun
TI  - [Immunocytochemistry of proteoglycan in dentin and odontoblasts].
PG  - 239-45
AB  - To clarify the distribution of proteoglycan (PG) and matrix metalloproteinase-3 
      (MMP-3) in the molar tooth germ dentin of rats, immunohistochemical investigation 
      was performed using a antibody 2-B-6 specific for chondroitin 4 sulfate (Ch4-S) 
      and dermatan sulfate (DS) of PG, antibody 3-B-3 specific for chondroitin 6 
      sulfate (Ch6-S) and chondroitin (Ch) of PG and anti-human-MMP3 antibody. 2-B-6 
      reacted with the predentin and dentinal tubules. However, there were no reactions 
      around the calcification front or in the calcified dentin matrix other than the 
      dentinal tubules. 3-B-3 and anti-MMP3 antibodies reacted with only the predentin. 
      These results showed that PG containing Ch4-S and DS was present in the dentinal 
      tubules, while PG containing Ch4-S, DS, Ch6-S and Ch as well as MMP-3 was present 
      in the predentin. It is suggested that PG of the predentin is degraded by MMP-3 
      at the calcification front. In undecalcified sections, the reaction of the 
      dentinal tubules with 2-B-6 was localized in electron-dense sites corresponding 
      to the peritubular dentin, suggesting that PG at this site binds to 
      hydroxyapatite. Furthermore, in odontoblasts, the localization of 2-B-6 was noted 
      in secretory granules distributed in the Golgi region and the odontoblastic 
      process. This suggests that PG is transported from the Golgi region to the 
      odontoblastic process in the form of secretory granules. Immunoreactive secretory 
      granules were also noted in the cellular process of the calcified dentin. PG of 
      the peritubular dentin may be secreted by these secretory granules in the 
      process.
FAU - Moriguchi, M
AU  - Moriguchi M
AD  - Department of Ultrastructural Science, Tokyo Dental College, Chiba, Japan.
FAU - Yamada, M
AU  - Yamada M
FAU - Yanagisawa, T
AU  - Yanagisawa T
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Kaibogaku Zasshi
JT  - Kaibogaku zasshi. Journal of anatomy
JID - 0413526
RN  - 0 (Proteoglycans)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Dentin/*chemistry
MH  - Immunohistochemistry
MH  - Matrix Metalloproteinase 3/analysis
MH  - Odontoblasts/*chemistry
MH  - Proteoglycans/*analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
PST - ppublish
SO  - Kaibogaku Zasshi. 1998 Jun;73(3):239-45.