PMID- 9712425
OWN - NLM
STAT- MEDLINE
DCOM- 19980821
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 29
IP  - 8
DP  - 1998 Aug
TI  - Analysis of hematologic diseases using conventional karyotyping, fluorescence in 
      situ hybridization (FISH), and comparative genomic hybridization (CGH).
PG  - 833-9
AB  - Comparative genomic hybridization (CGH) has been proven to be an important tool 
      in interphase cytogenetics of solid tumors. Although, because of methodological 
      implications, balanced aberrations are not detected by CGH, the technique has 
      uncovered a variety of new and interesting imbalanced karyotype changes. However, 
      only a few studies deal with its application to hematologic disorders, although 
      this is a main topic of cytogenetics. The aim of our study was, therefore, to 
      evaluate the usefulness of CGH in the examination of hematologic neoplasms. For 
      this purpose, bone marrow aspirates of 33 patients with different hematologic 
      disorders were examined with CGH and the results compared with conventional 
      cytogenetics (CC) and fluorescence in situ hybridization (FISH). CGH showed 
      chromosome changes in 8 of 33 cases. CC found balanced aberrations in 4 of 33 and 
      unbalanced changes in 9 of 33 samples. Differences between CGH and CC in 
      unbalanced aberrations were seen in four cases. In these samples, either the 
      number of aberrant cells found by CC was low and, therefore, difficult to detect 
      by CGH, or complex aberrations in different cell clones as seen in CC were lumped 
      together as one karyotype by CGH. In one sample, CC was not capable of giving any 
      results at all, whereas CGH showed trisomy 8. CGH was also helpful in defining 
      the bands involved in the structural aberrations, which was difficult by CC in 
      some cases because of the low quality of metaphase spreads. All results obtained 
      by CGH were confirmed by FISH, whereas CC and FISH were discordant in one case. 
      Although CGH was not able to detect all aberrations, it gave important additional 
      information for the correct localization of the aberrations found in CC, and it 
      was most helpful in samples not processed successfully in CC. These advantages 
      would open up a new field of application for CGH not only for research, but also 
      for diagnostic purposes.
FAU - Wilkens, L
AU  - Wilkens L
AD  - Pathologisches Institut der Medizinischen Hochschule, Hannover, Germany.
FAU - Tchinda, J
AU  - Tchinda J
FAU - Burkhardt, D
AU  - Burkhardt D
FAU - Nolte, M
AU  - Nolte M
FAU - Werner, M
AU  - Werner M
FAU - Georgii, A
AU  - Georgii A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow
MH  - Chromosome Aberrations/*genetics
MH  - Chronic Disease
MH  - DNA, Neoplasm/*analysis/isolation & purification
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics/pathology
MH  - Lymphoma, Non-Hodgkin/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/pathology
MH  - Myeloproliferative Disorders/*genetics/pathology
MH  - Nucleic Acid Hybridization
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - S0046-8177(98)90453-1 [pii]
AID - 10.1016/s0046-8177(98)90453-1 [doi]
PST - ppublish
SO  - Hum Pathol. 1998 Aug;29(8):833-9. doi: 10.1016/s0046-8177(98)90453-1.