PMID- 9712521
OWN - NLM
STAT- MEDLINE
DCOM- 19981110
LR  - 20090929
IS  - 0214-6282 (Print)
IS  - 0214-6282 (Linking)
VI  - 42
IP  - 5
DP  - 1998 Jul
TI  - Tumor necrosis factor-alpha inhibits epithelial differentiation and morphogenesis 
      in the mouse metanephric kidney in vitro.
PG  - 663-74
AB  - Tumor necrosis factor-alpha (TNF-alpha), an inflammatory cytokine, has diverse 
      actions both within and outside the immune system and has been implicated in the 
      etiology of a wide range of pathological conditions. Evidence is accumulating 
      that it may also have important roles in the normal development of the embryo. In 
      this study we demonstrated that the addition of recombinant TNF-alpha to 
      metanephric organ culture induced a dose dependent and reversible decrease in 
      growth and development, with inhibition of ureteric bud branching and nephron 
      formation beyond the condensate stage and despite appropriate expression of the 
      transcription factor pax-2. TNF-alpha also increased the point prevalence of 
      apoptosis after only 1 day of culture. We also noted that macrophages were 
      present in renal rudiments at the inception of nephrogenesis and their numbers 
      significantly increased during the culture period. This effect was enhanced by 
      TNF-alpha. We have also demonstrated expression of mRNAs for TNF-alpha and its 
      receptors in whole mouse metanephroi from the inception of renal development. 
      TNF-alpha protein was also detected, predominantly at mesenchymal/epithelial 
      interfaces. In addition, TNF-alpha mRNA and protein were expressed by clonal 
      renal mesenchymal cells in vitro, suggesting that these cells are a source of 
      TNF-alpha in vivo.
FAU - Cale, C M
AU  - Cale CM
AD  - Developmental Biology and Nephrourology, Institute of Child Health, London, 
      United Kingdom. ccale@ich.ucl.ac.uk
FAU - Klein, N J
AU  - Klein NJ
FAU - Morgan, G
AU  - Morgan G
FAU - Woolf, A S
AU  - Woolf AS
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Int J Dev Biol
JT  - The International journal of developmental biology
JID - 8917470
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Differentiation/drug effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Gene Expression Regulation, Developmental
MH  - Kidney/cytology/*embryology
MH  - Mice
MH  - Morphogenesis
MH  - Nephrons/cytology/embryology
MH  - Organ Culture Techniques
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/*genetics/pharmacology
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
PST - ppublish
SO  - Int J Dev Biol. 1998 Jul;42(5):663-74.