PMID- 9712699
OWN - NLM
STAT- MEDLINE
DCOM- 19980911
LR  - 20220331
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 249
IP  - 2
DP  - 1998 Aug 19
TI  - Proliferation and differentiation of human osteoblastic cells associated with 
      differential activation of MAP kinases in response to epidermal growth factor, 
      hypoxia, and mechanical stress in vitro.
PG  - 350-4
AB  - In an attempt to elucidate the specificity of pathways from environmental stress 
      to cellular outcome via mitogen activated protein kinases (MAPKs) activation, we 
      examined the responsiveness of cultured human osteoblastic periodontal ligament 
      (PDL) cells to epidermal growth factor (EGF), hypoxia, and mechanical stress, in 
      terms of cell proliferation, differentiation, and associated activation of three 
      different types of MAPK. Cell proliferation was promoted in the presence of 
      10ng/ml of EGF or in hypoxic conditions (5% O2), whereas it was inhibited by 
      cyclic stretch (9% strain, 6 cycles/min), which was used as a model of mechanical 
      stress. Conversely, the alkaline phosphatase activity, a marker for osteoblastic 
      differentiation of the cells, was increased by cyclic stretch but decreased by 
      EGF and hypoxia. The mitogenic response of PDL cells to EGF or hypoxia was 
      associated with the selective phosphorylation and activation of 
      extracellular-related kinase (ERK) 1/2, while phosphorylation and activation of 
      c-Jun N-terminal kinase (JNK) was observed in mechanical stretch loaded cells. No 
      such changes were seen in p38 protein. These findings suggested that 
      stress-responsive changes in proliferation and osteoblastic differentiation of 
      PDL cells are selectively mediated by ERK 1/2 and by JNK, respectively, and that 
      a balance between these two pathways determines the cell fate.
CI  - Copyright 1998 Academic Press.
FAU - Matsuda, N
AU  - Matsuda N
AD  - Nagasaki University Radioisotope Center, Nagasaki, Japan.
FAU - Morita, N
AU  - Morita N
FAU - Matsuda, K
AU  - Matsuda K
FAU - Watanabe, M
AU  - Watanabe M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Culture Media)
RN  - 0 (Nerve Tissue Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
MH  - *Cell Differentiation
MH  - *Cell Division
MH  - *Cell Hypoxia
MH  - Cells, Cultured
MH  - Culture Media
MH  - Enzyme Activation
MH  - Epidermal Growth Factor/*pharmacology
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Osteoblasts/*cytology/enzymology
MH  - Periodontal Ligament
MH  - Phosphorylation
MH  - Stress, Mechanical
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - S0006-291X(98)99151-3 [pii]
AID - 10.1006/bbrc.1998.9151 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1998 Aug 19;249(2):350-4. doi: 
      10.1006/bbrc.1998.9151.