PMID- 9713468
OWN - NLM
STAT- MEDLINE
DCOM- 19980827
LR  - 20151119
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 18
IP  - 4B
DP  - 1998 Jul-Aug
TI  - Prognostic significance of different biological markers (DNA index, PCNA index, 
      apoptosis, p53, karyotype) in 126 adenocarcinoma gastric biopsies.
PG  - 2819-25
AB  - BACKGROUND: The different clinical evolution of gastric adenocarcinomas with the 
      same clinico-pathologic characteristics prompted the authors to investigate the 
      prognostic significance of different biological markers. METHODS: One hundred 
      twenty-six preoperative cancer gastric biopsies, selected according the stage 
      evaluated after following gastrectomy, were examined for DNA content by means the 
      static cytometry, proliferating cell nuclear antigen (PCNA), p53 mutation, 
      apoptosis by immunohistochemistry and karyotype using fluorescence in situ 
      hybridization (FISH) techniques. RESULTS: The gastric cancers, ail belonging at 
      stage III, were adenocarcinomas histologically typed as: 26 well differentiated 
      (G1), 45 moderately differentiated (G2), 43 poorly differentiated (G3) and 12 of 
      undifferentiated type with signet-ring cells. The tumours showed a prevalence of 
      diploidy (68%), a mean PCNA-LI of 4.8%, trisomy of chromosome 7 in 40% of the 
      cases, low presence of apoptosis (30%) and p53 mutation (17%). Only apoptosis was 
      significantly correlated to histological diagnosis (p = 0.009). A multivariate 
      analysis showed that the DNA content and PCNA-LI were the only independent 
      prognostic markers for survival (p = 0.005 and p = 0.0002 respectively). 
      CONCLUSIONS: The evaluation of these two biological variables, especially of the 
      PCNA index, on gastric cancer biopsies may be useful in predicting the 
      aggressiveness of each tumor and in identifying patients in need of additional 
      perioperative therapies.
FAU - Matturri, L
AU  - Matturri L
AD  - Institute of Pathology, University of Milan, IRCCS, Opedale Maggiore di Milano, 
      Italy. MATTURRI@IMIUCCA.CSI.UNIMI.IT
FAU - Biondo, B
AU  - Biondo B
FAU - Cazzullo, A
AU  - Cazzullo A
FAU - Colombo, B
AU  - Colombo B
FAU - Giordano, F
AU  - Giordano F
FAU - Guarino, M
AU  - Guarino M
FAU - Pallotti, F
AU  - Pallotti F
FAU - Turconi, P
AU  - Turconi P
FAU - Lavezzi, A M
AU  - Lavezzi AM
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adenocarcinoma/*diagnosis/genetics/metabolism/pathology
MH  - Aged
MH  - Aged, 80 and over
MH  - Apoptosis
MH  - *Biomarkers, Tumor
MH  - Chromosomes, Human, Pair 7
MH  - Diploidy
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Stomach Neoplasms/*diagnosis/genetics/metabolism/pathology
MH  - Tumor Suppressor Protein p53/genetics/metabolism
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1998 Jul-Aug;18(4B):2819-25.