PMID- 9713594
OWN - NLM
STAT- MEDLINE
DCOM- 19980831
LR  - 20190503
IS  - 1366-8714 (Print)
IS  - 1472-4154 (Electronic)
IS  - 1366-8714 (Linking)
VI  - 51
IP  - 2
DP  - 1998 Apr
TI  - AgNORs in the myocardium in ischaemic heart disease complicated by heart failure: 
      a postmortem study.
PG  - 102-4
AB  - AIM: To evaluate the interphase ribosomal RNA cistron activity of cardiomyocytes 
      in case with ischaemic heart disease complicated by heart failure. METHODS: 
      Nucleoli were investigated in postmortem myocardium samples from 31 cases with 
      ischaemic heart disease (mean (SEM), 57.4 (6.5) years) with or without severe 
      heart failure (18 and 13, respectively) and from eight healthy people who died in 
      accidents (mean (SEM) 25.3 (4.0) years). Myocardium obtained within one hour 
      after death was frozen in liquid nitrogen. Silver staining for nucleolar 
      organiser regions (AgNOR) was performed with a standard procedure and the mean 
      score obtained. On the basis of these data, the average number of AgNORs per 
      nucleus was determined. The Student's t test was used to compare groups. RESULTS: 
      Compared with controls, the mean numbers of AgNORs per nucleus in cardiomyocytes 
      from ischaemic heart disease patients not complicated with severe heart failure 
      were higher (8.0 v 9.9; p < 0.05), but cases with severe heart failure had a 
      progressive decrease in cardiomyocyte AgNORs. A difference in AgNOR numbers 
      between cases with different ischaemic heart disease courses was found when cases 
      with the same New York Heart Association (NYHA) functional stage III of heart 
      failure were studied. CONCLUSIONS: The significant decrease of AgNORs in 
      cardiomyocytes from cases with severe ischaemic heart disease complicated by 
      heart failure seems to be connected with cardiomyocyte adaptation (a variant of 
      hibernation) to a diminished circulation that, hypothetically, may affect the 
      level of ribosomal RNA synthesis.
FAU - Mamaev, N N
AU  - Mamaev NN
AD  - Division of Cardiology, Pavlov Medical University of St Petersburg, Russia.
FAU - Gudkova, A Y
AU  - Gudkova AY
FAU - Amineva, K K
AU  - Amineva KK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mol Pathol
JT  - Molecular pathology : MP
JID - 9706282
SB  - IM
MH  - Adult
MH  - Female
MH  - Heart Failure/etiology/*pathology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Myocardial Ischemia/complications/*pathology
MH  - Myocardium/*ultrastructure
MH  - Nucleolus Organizer Region/*pathology
MH  - Silver Staining
PMC - PMC395618
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
PMCR- 1998/04/01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
PHST- 1998/04/01 00:00 [pmc-release]
AID - 10.1136/mp.51.2.102 [doi]
PST - ppublish
SO  - Mol Pathol. 1998 Apr;51(2):102-4. doi: 10.1136/mp.51.2.102.