PMID- 9713764
OWN - NLM
STAT- MEDLINE
DCOM- 19981022
LR  - 20230307
IS  - 0362-028X (Print)
IS  - 0362-028X (Linking)
VI  - 61
IP  - 8
DP  - 1998 Aug
TI  - Characteristics of the LacTek test as applied to tissue samples: assessment of 
      performance using incurred field samples.
PG  - 1018-22
AB  - The Lactek test, marketed for antimicrobial residue detection in milk, was 
      validated for the detection of antimicrobial residues in tissues. A previous 
      study found that the LacTek test could confidently identify tissue samples spiked 
      with antimicrobial residues. However, the test could not reliably distinguish 
      violative from nonviolative spiked samples relative to Canadian maximum residue 
      limits (MRLs). The objectives of this study were to assess and compare the 
      performance of the LacTek tests for beta-lactams, tetracyclines, gentamicin, and 
      sulfamethazine on samples containing naturally incurred residues by running the 
      test in parallel with the standard microbial inhibition test (MIT) presently used 
      for the routine testing of tissues at our facility and to assess the agreement 
      with high pressure liquid chromatographic (HPLC) determinative methods. Parallel 
      testing with the official MIT found that the Lactek tests could be confidently 
      used for testing tissue samples containing incurred residues. Among 1,008 
      MIT-positive samples, the LacTek test found that 90% contained beta-lactams 
      and/or tetracyclines. A further 7.3% of violative residues could not be 
      identified to an antimicrobial class. In addition, 9% of samples testing negative 
      on the MIT were found to contain an antimicrobial residue by the LacTek tests. 
      Comparative testing with HPLC methods found that there was very good agreement 
      between the two tests and that most violations were due to penicillin G and 
      oxytetracycline. Although the LacTek test cannot be used to distinguish violative 
      from nonviolative residue levels, it does offer several advantages over the 
      present MIT. These include speed, ease of use, the ability to identify residues 
      to a specific class, and an improved sensitivity at the MRL level for the most 
      commonly found antimicrobials in tissue.
FAU - Mitchell, J M
AU  - Mitchell JM
AD  - University of Guelph, Ontario, Canada. mmitchel@lsd.uoguelph.ca
FAU - McNab, W B
AU  - McNab WB
FAU - Yee, A J
AU  - Yee AJ
FAU - Griffiths, M W
AU  - Griffiths MW
FAU - McEwen, S A
AU  - McEwen SA
FAU - Spilsbury, L
AU  - Spilsbury L
FAU - Boison, J O
AU  - Boison JO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - *Food Analysis
MH  - Immunoenzyme Techniques
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - S0362-028X(22)01647-7 [pii]
AID - 10.4315/0362-028x-61.8.1018 [doi]
PST - ppublish
SO  - J Food Prot. 1998 Aug;61(8):1018-22. doi: 10.4315/0362-028x-61.8.1018.