PMID- 9713936
OWN - NLM
STAT- MEDLINE
DCOM- 19981022
LR  - 20211216
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 16
IP  - 17
DP  - 1998 Oct
TI  - Display of an inhibin epitope in a surface-exposed loop of the E. coli 
      heat-labile enterotoxin B subunit.
PG  - 1611-9
AB  - In vitro gene manipulation was used to develop a novel chimeric antigen 
      consisting of the non-toxic B subunit (EtxB) of an E. coli enterotoxin and the 
      first 14 N-terminal amino acid residues of the carboxy-terminal portion of the 
      alpha subunit of bovine inhibin (bINH1-14). Rabbits immunized subcutaneously 
      (s.c.) or intravenously (i.v.) with EtxB::bINH1-14, with or without Freund's 
      adjuvant, developed significant titres of antibodies that recognized an inhibin 
      peptide fragment containing bINH1-14, native inhibins, and EtxB during separate 
      enzyme-linked immunosorbent assay (ELISA). Passive immunization of mice with the 
      rabbit anti-EtxB::bINH1-14 serum increased concentrations of follicle-stimulating 
      hormone (FSH) in serum twofold compared with controls, whereas serum 
      concentrations of luteinizing hormone (LH) were unaltered. Since FSH is the 
      primary hormone from the pituitary gland that stimulates ovarian follicle growth 
      and spermatogenesis, the results of this study demonstrate that EtxB::bINH1-14 
      has potential as antigen for development of inhibin-based fertility vaccines.
FAU - Sewani, C R
AU  - Sewani CR
AD  - Department of Physiology, Michigan State University, East Lansing 48824-1312, 
      USA.
FAU - Bagdasarian, M M
AU  - Bagdasarian MM
FAU - Ireland, J J
AU  - Ireland JJ
FAU - Bagdasarian, M
AU  - Bagdasarian M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Epitopes)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - 57285-09-3 (Inhibins)
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Toxins/*immunology
MH  - Base Sequence
MH  - Cattle
MH  - Enterotoxins/*immunology
MH  - Epitopes
MH  - Escherichia coli/*immunology
MH  - *Escherichia coli Proteins
MH  - Female
MH  - Humans
MH  - Immunization
MH  - Inhibins/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Rabbits
MH  - Recombinant Fusion Proteins/*immunology
MH  - Vaccines, Synthetic/*immunology
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - S0264-410X(98)00056-5 [pii]
AID - 10.1016/s0264-410x(98)00056-5 [doi]
PST - ppublish
SO  - Vaccine. 1998 Oct;16(17):1611-9. doi: 10.1016/s0264-410x(98)00056-5.