PMID- 9713991
OWN - NLM
STAT- MEDLINE
DCOM- 19980930
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 23
IP  - 1
DP  - 1998 Sep
TI  - Rearrangement between the MYH11 gene at 16p13 and D12S158 at 12p13 in a case of 
      acute myeloid leukemia M1 (AML-M1).
PG  - 10-5
AB  - A case of acute myeloid leukemia (AML) M1 with bone marrow eosinophilia was 
      characterized by cytogenetics and fluorescence in situ hybridization (FISH). A 
      complex karyotype including a der(12)t(12;17)(p12-13;q11) and a 
      der(16)t(16;20)(p13;p11) was found at diagnosis. FISH studies with probes for 
      chromosome 16 and for the short arm of chromosome 12 showed even more complex 
      rearrangements. Analysis with a panel of probes for 12p showed that D12S158 
      spanned the breakpoint on the der(12). Unexpectedly, FISH signals were found on 
      the der(12) and on the der(6) at band p13, the site of juxtaposition between the 
      short arm of chromosome 16 and chromosome 20. Moreover, both YAC 854E2, 
      containing the MYH11 gene, and cosmid ZIT133, encompassing the MYH11 breakpoint 
      in inv(16) and t(16;16) of AML-M4 with eosinophilia, demonstrated fluorescent 
      signals on the normal 16, on the der(16), and on the der(12). These data clearly 
      support a reciprocal exchange between D12S158 at 12p13.3 and the MYH11 gene at 
      16p13. In addition, experiments with two PAC clones for the CBFB gene at 16q22 
      excluded the presence of a masked inv(16). An interstitial deletion, independent 
      from the translocation and flanked by VWF and KRAS2, was also detected on the 
      der(12).
FAU - La Starza, R
AU  - La Starza R
AD  - Hematology and Bone Marrow Transplantation Unit, University of Perugia, Italy.
FAU - Wlodarska, I
AU  - Wlodarska I
FAU - Matteucci, C
AU  - Matteucci C
FAU - Falzetti, D
AU  - Falzetti D
FAU - Baens, M
AU  - Baens M
FAU - Martelli, M F
AU  - Martelli MF
FAU - Van den Berghe, H
AU  - Van den Berghe H
FAU - Marynen, P
AU  - Marynen P
FAU - Mecucci, C
AU  - Mecucci C
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Transcription Factors)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Aged
MH  - Chromosome Inversion
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Chromosomes, Human, Pair 16/*genetics
MH  - Genes/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Microsatellite Repeats/genetics
MH  - Myosin Heavy Chains/*genetics
MH  - Transcription Factors/genetics
MH  - Translocation, Genetic
EDAT- 1998/08/26 02:24
MHDA- 2000/06/20 09:00
CRDT- 1998/08/26 02:24
PHST- 1998/08/26 02:24 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/08/26 02:24 [entrez]
AID - 10.1002/(SICI)1098-2264(199809)23:1<10::AID-GCC2>3.0.CO;2-9 [pii]
AID - 10.1002/(sici)1098-2264(199809)23:1<10::aid-gcc2>3.3.co;2-7 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1998 Sep;23(1):10-5. doi: 
      10.1002/(sici)1098-2264(199809)23:1<10::aid-gcc2>3.3.co;2-7.