PMID- 9714185
OWN - NLM
STAT- MEDLINE
DCOM- 19980831
LR  - 20131121
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 78
IP  - 8
DP  - 1998 Aug
TI  - Production and regulation of monocyte chemoattractant protein-1 in 
      lipopolysaccharide- or monosodium urate crystal-induced arthritis in rabbits: 
      roles of tumor necrosis factor alpha, interleukin-1, and interleukin-8.
PG  - 973-85
AB  - The production of monocyte chemoattractant protein-1 (MCP-1) and its regulation 
      by TNFalpha, IL-1, and IL-8 were investigated in two rabbit models of arthritis 
      induced by intra-articular injection of lipopolysaccharide (LPS) or monosodium 
      urate (MSU) crystals. We first prepared recombinant rabbit MCP-1 and antibodies 
      and then developed an immunoassay. The immunoassay detected 3 pg/ml rabbit MCP-1 
      and did not cross-react with other rabbit chemokines such as IL-8 or GRO. MCP-1 
      was first detected in synovial fluid (SF) at 1 hour, and peaked at 4 or 2 hours 
      after the injection of LPS or MSU crystals, respectively. Immunohistochemically, 
      MCP-1 was detected in synovial lining cells and infiltrating neutrophils. The 
      amounts of MCP-1 detected in SF from neutrophil-depleted rabbits were similar to 
      those in normal rabbits, suggesting that synovial lining cells were the main 
      source of MCP-1 detected in SF. The peak level of MCP-1 in SF after LPS-injection 
      was inhibited by 57% with anti-TNFalpha mAb and by 41% with IL-1 receptor 
      antagonist (IL-1Ra). Coadministration of anti-TNFalpha mAb and IL-1Ra inhibited 
      90% of MCP-1 production. In contrast, the peak level of MCP-1 in SF after MSU 
      crystal-injection was not affected by any cytokine inhibitor, but was reduced by 
      52% with coadministration of anti-TNFalpha mAb and IL-1Ra. Anti-IL-8 IgG had no 
      effect on the production of MCP-1 in either model. Thus, the production of MCP-1 
      in LPS-induced arthritis was mostly regulated by TNFalpha and IL-1, whereas half 
      the extent of MCP-1 production in MSU crystal-induced arthritis was independent 
      of TNFalpha or IL-1. IL-8 does not seem to regulate the production of MCP-1 in SF 
      either directly or indirectly. Finally, administration of neutralizing anti-MCP-1 
      antibody inhibited LPS- and MSU crystal-induced monocyte infiltration by 58.4% 
      and 44.9%, respectively, suggesting that synovial production of MCP-1 plays an 
      important role in the recruitment of monocytes in these arthritis models.
FAU - Matsukawa, A
AU  - Matsukawa A
AD  - Department of Pathology, Kumamoto University School of Medicine, Honjo, Japan.
FAU - Miyazaki, S
AU  - Miyazaki S
FAU - Maeda, T
AU  - Maeda T
FAU - Tanase, S
AU  - Tanase S
FAU - Feng, L
AU  - Feng L
FAU - Ohkawara, S
AU  - Ohkawara S
FAU - Yoshinaga, M
AU  - Yoshinaga M
FAU - Yoshimura, T
AU  - Yoshimura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immune Sera)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 268B43MJ25 (Uric Acid)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/biosynthesis
MH  - Arthritis/chemically induced/*metabolism/pathology
MH  - COS Cells
MH  - Cell Movement/immunology
MH  - Chemokine CCL2/analysis/*biosynthesis/genetics/immunology
MH  - Crystallization
MH  - Female
MH  - Fluoroimmunoassay
MH  - Immune Sera/administration & dosage/biosynthesis
MH  - Injections, Intra-Articular
MH  - Injections, Intravenous
MH  - Interleukin-1/*physiology
MH  - Interleukin-8/*physiology
MH  - Knee Joint/pathology
MH  - Lipopolysaccharides/*toxicity
MH  - Monocytes/pathology
MH  - Rabbits
MH  - Recombinant Proteins/analysis/biosynthesis/immunology
MH  - Synovial Fluid/chemistry
MH  - Tumor Necrosis Factor-alpha/*physiology
MH  - Uric Acid/*toxicity
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1998 Aug;78(8):973-85.