PMID- 9714986
OWN - NLM
STAT- MEDLINE
DCOM- 19980909
LR  - 20191102
IS  - 1341-8076 (Print)
IS  - 1341-8076 (Linking)
VI  - 24
IP  - 3
DP  - 1998 Jun
TI  - Ultrashort-time FISH for identification of the sex of peripheral-blood 
      lymphocytes and preimplantation embryos.
PG  - 167-71
AB  - OBJECTIVE: The aim of this study was to shorten the time required for sex 
      determination using peripheral-blood lymphocytes and blastomeres of human 
      preimplantation embryos by fluorescence in situ hybridization (FISH). METHODS: 
      Peripheral-blood lymphocytes from a male and a female, and 8 blastomeres from 2 
      preimplantation embryos were analyzed by FISH. The probes used were DYZI and 
      DXZI. The hybridization time was shortened from overnight to 30 minutes or 5 
      minutes, and the accuracy of FISH was compared between 5-minute, 30-minute and 
      overnight hybridization. RESULTS: The accuracy of the identification of the sex 
      of lymphocytes by 5-minute hybridization FISH was more than 90%, and was the same 
      as that achieved by 30-minute- and overnight hybridization FISH. In 5-minute 
      hybridization FISH, the entire process was only 63 minutes. Of the 8 blastomeres, 
      it was possible to identify the sex of 5 and was impossible to determine the sex 
      of 1, with 2 being lost during the procedure. CONCLUSIONS: We have established on 
      ultrashort-time FISH method for sex determination that takes only 63 minutes.
FAU - Ikeda, T
AU  - Ikeda T
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Kagoshima 
      University, Japan.
FAU - Fujino, T
AU  - Fujino T
FAU - Nagata, Y
AU  - Nagata Y
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - J Obstet Gynaecol Res
JT  - The journal of obstetrics and gynaecology research
JID - 9612761
SB  - IM
MH  - *Blastocyst
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Lymphocytes
MH  - Male
MH  - Sex Determination Analysis/*methods
MH  - Time Factors
EDAT- 1998/08/26 00:00
MHDA- 1998/08/26 00:01
CRDT- 1998/08/26 00:00
PHST- 1998/08/26 00:00 [pubmed]
PHST- 1998/08/26 00:01 [medline]
PHST- 1998/08/26 00:00 [entrez]
AID - 10.1111/j.1447-0756.1998.tb00071.x [doi]
PST - ppublish
SO  - J Obstet Gynaecol Res. 1998 Jun;24(3):167-71. doi: 
      10.1111/j.1447-0756.1998.tb00071.x.