PMID- 9719229
OWN - NLM
STAT- MEDLINE
DCOM- 19981022
LR  - 20190921
IS  - 0944-1174 (Print)
IS  - 0944-1174 (Linking)
VI  - 33
IP  - 4
DP  - 1998 Aug
TI  - No Mycobacterium paratuberculosis detected in intestinal tissue, including 
      Peyer's patches and lymph follicles, of Crohn's disease.
PG  - 482-7
AB  - To clarify the etiologic significance of Mycobacterium paratuberculosis in 
      Crohn's disease, we investigated whether M. paratuberculosis was detected in 
      intestinal tissues, including Peyer's patches, where M. paratuberculosis invades, 
      and colonic lymph follicles, where early lesions appear. Fifty-one samples of 
      intestinal tissues, either therapeutically resected or biopsied, including 34 
      specimens from 30 patients with Crohn's disease, were studied. Four Peyer's 
      patches and eight lymph follicles were included in the intestinal tissue samples 
      of Crohn's disease. They were visualized by acetic acid fixation. DNA extracted 
      from intestinal tissues by proteinase K treatment was used for nested polymerase 
      chain reaction (PCR) for detection of IS900, which is specific for M. 
      paratuberculosis. PCR products were analyzed by agarose gel electrophoresis and 
      subsequent Southern blot analysis. Our amplification system could detect 7.5 fg 
      of M. paratuberculosis DNA. None of the tissue samples showed positive IS900 
      amplification, whereas they all showed amplification of the positive control 
      human leukocyte antigen (HLA)-DQA DNA. Spiked experiments of tissue samples with 
      M. paratuberculosis demonstrated that inhibitors of IS900 amplification were not 
      present in the samples. Our study does not support the etiologic significance of 
      M. paratuberculosis in Crohn's disease.
FAU - Chiba, M
AU  - Chiba M
AD  - First Department of Internal Medicine, Akita University School of Medicine, 
      Japan.
FAU - Fukushima, T
AU  - Fukushima T
FAU - Horie, Y
AU  - Horie Y
FAU - Iizuka, M
AU  - Iizuka M
FAU - Masamune, O
AU  - Masamune O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Gastroenterol
JT  - Journal of gastroenterology
JID - 9430794
RN  - 0 (DNA Primers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Blotting, Southern
MH  - Child
MH  - Colitis, Ulcerative/*microbiology
MH  - Crohn Disease/*microbiology
MH  - DNA Primers
MH  - Electrophoresis
MH  - Female
MH  - Humans
MH  - Intestines/*microbiology
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium avium subsp. paratuberculosis/*isolation & purification
MH  - Peyer's Patches/*microbiology
MH  - Polymerase Chain Reaction
MH  - Specimen Handling
EDAT- 1998/08/27 00:00
MHDA- 1998/08/27 00:01
CRDT- 1998/08/27 00:00
PHST- 1998/08/27 00:00 [pubmed]
PHST- 1998/08/27 00:01 [medline]
PHST- 1998/08/27 00:00 [entrez]
AID - 10.1007/s005350050119 [doi]
PST - ppublish
SO  - J Gastroenterol. 1998 Aug;33(4):482-7. doi: 10.1007/s005350050119.