PMID- 9720181
OWN - NLM
STAT- MEDLINE
DCOM- 19980924
LR  - 20190915
IS  - 0268-9146 (Print)
IS  - 0268-9146 (Linking)
VI  - 29
IP  - 3
DP  - 1998 Jun
TI  - Construction of a bovine yeast artificial chromosome (YAC) library.
PG  - 216-9
AB  - We have constructed a bovine yeast artificial chromosome (YAC) library to provide 
      a common resource for bovine genome research. We used leukocytes of a Japanese 
      black bull (Bos taurus) as the DNA source, AB1380 for the yeast host, and pYAC4 
      for the vector. The library consists of 24,576 clones arranged in 256 96-well 
      microtiter plates. An average insert size estimated from the analysis of 251 
      randomly selected clones was 480 kb. The rate of chimeric YACs evaluated by 
      fluorescence in situ hybridization (FISH) analysis of 44 randomly selected clones 
      was 36.4%. To estimate the number of genome equivalents, PCR-based screening was 
      performed with 48 primer pairs and isolated 3.2 clones on average. In order to 
      provide broad access for the scientific community, this library has been 
      incorporated into the Reference Library system which provides high density 
      filters for colony hybridization screening and a common database of the library.
FAU - Takeda, H
AU  - Takeda H
AD  - Shirakawa Institute of Animal Genetics, Fukushima, Japan.
FAU - Yamakuchi, H
AU  - Yamakuchi H
FAU - Ihara, N
AU  - Ihara N
FAU - Hara, K
AU  - Hara K
FAU - Watanabe, T
AU  - Watanabe T
FAU - Sugimoto, Y
AU  - Sugimoto Y
FAU - Oshiro, T
AU  - Oshiro T
FAU - Kishine, H
AU  - Kishine H
FAU - Kano, Y
AU  - Kano Y
FAU - Kohno, K
AU  - Kohno K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cattle/*genetics
MH  - Chimera
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Cloning, Molecular
MH  - DNA/analysis/genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - *Gene Library
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Japan
MH  - Male
EDAT- 1998/08/28 00:00
MHDA- 1998/08/28 00:01
CRDT- 1998/08/28 00:00
PHST- 1998/08/28 00:00 [pubmed]
PHST- 1998/08/28 00:01 [medline]
PHST- 1998/08/28 00:00 [entrez]
AID - 10.1046/j.1365-2052.1998.00325.x [doi]
PST - ppublish
SO  - Anim Genet. 1998 Jun;29(3):216-9. doi: 10.1046/j.1365-2052.1998.00325.x.