PMID- 9720889
OWN - NLM
STAT- MEDLINE
DCOM- 19981130
LR  - 20190815
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 19
IP  - 14
DP  - 1998 Jul
TI  - Effects of biodegradable polymer particles on rat marrow-derived stromal 
      osteoblasts in vitro.
PG  - 1255-68
AB  - Effects of biodegradable particles of poly(L-lactic acid) (PLLA) and 
      poly(DL-lactic-co-glycolic acid) (PLGA) 50/50 with diameter ranging from 1.0 to 
      1.5 microm on rat marrow stromal osteoblasts in vitro have been investigated over 
      a period of 28 days. This study examined the effects of three particle 
      parameters, concentration, polymer molecular weight, and composition, on 
      osteoblast proliferation and function. Cell cultures were challenged with 
      particles at two different time points: upon cell seeding (Day 1), and after 
      cells had begun to establish their own mineralized extracellular matrix (Day 14). 
      The most significant trend observed in those cultures challenged with particles 
      beginning on Day 1 was due to increasing the concentration of particles, 
      resulting in decreased [3H]-thymidine incorporation, cell count, and 
      mineralization. Those cultures challenged with particles beginning on Day 14 were 
      significantly more mineralized than those challenged with particles beginning on 
      Day 1. In addition, increasing osteocalcin secretion confirmed the osteoblastic 
      phenotype of the derived stromal cells. These studies suggest that the particles 
      may affect the bone remodeling process surrounding a degrading implant by direct 
      interaction with osteoblasts in addition to their indirect contributions to the 
      inflammatory mechanism via mediators secreted by macrophages upon their 
      phagocytosis.
FAU - Wake, M C
AU  - Wake MC
AD  - Institute of Biosciences and Bioengineering and Department of Chemical 
      Engineering, Rice University, Houston, TX 77251-1892, USA.
FAU - Gerecht, P D
AU  - Gerecht PD
FAU - Lu, L
AU  - Lu L
FAU - Mikos, A G
AU  - Mikos AG
LA  - eng
GR  - R29-AR42639/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 104982-03-8 (Osteocalcin)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 459TN2L5F5 (poly(lactide))
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biocompatible Materials/*pharmacology
MH  - Biodegradation, Environmental
MH  - Bone Marrow Cells/drug effects
MH  - Bone Remodeling
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Lactic Acid/*pharmacology
MH  - Male
MH  - Molecular Weight
MH  - Osteoblasts/*drug effects
MH  - Osteocalcin/metabolism
MH  - Particle Size
MH  - Polyesters
MH  - Polyglycolic Acid/*pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*pharmacology
MH  - Prostheses and Implants
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NASA
OT  - Non-programmatic
EDAT- 1998/08/28 00:00
MHDA- 1998/08/28 00:01
CRDT- 1998/08/28 00:00
PHST- 1998/08/28 00:00 [pubmed]
PHST- 1998/08/28 00:01 [medline]
PHST- 1998/08/28 00:00 [entrez]
AID - S0142961298000222 [pii]
AID - 10.1016/s0142-9612(98)00022-2 [doi]
PST - ppublish
SO  - Biomaterials. 1998 Jul;19(14):1255-68. doi: 10.1016/s0142-9612(98)00022-2.