PMID- 9721806
OWN - NLM
STAT- MEDLINE
DCOM- 19980902
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 66
IP  - 3
DP  - 1998 Aug 15
TI  - Butylated hydroxytoluene and N-acetylcysteine attenuates tumor necrosis 
      factor-alpha (TNF-alpha) secretion and TNF-alpha mRNA expression in alveolar 
      macrophages from human lung transplant recipients in vitro.
PG  - 364-9
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is a polypeptide cytokine 
      principally produced by macrophages/monocytes and commonly associated with 
      inflammatory conditions. The present study was designed to investigate whether 
      the antioxidants butylated hydroxytoluene (BHT) and N-acetylcysteine (NAC) 
      modified TNF-alpha production in stimulated and unstimulated alveolar macrophages 
      from lung transplant recipients in vitro. METHODS: The effects of BHT and NAC on 
      TNF-alpha production were studied both with and without lipopolysaccharide (LPS) 
      activation of alveolar macrophages from bronchoalveolar lavage fluid. TNF-alpha 
      was quantitated in cell culture medium using an enzyme-linked immunosorbent 
      assay. TNF-alpha mRNA expression was analyzed by quantitative reverse 
      transcription-polymerase chain reaction on total RNA extracted from the incubated 
      alveolar macrophages. RESULTS: In unstimulated alveolar macrophages, TNF-alpha 
      levels were significantly reduced by incubation with BHT or NAC. When alveolar 
      macrophages from patients with cytomegalovirus infection were incubated with BHT, 
      TNF-alpha secretion was significantly lowered. A significant reduction of 
      TNF-alpha levels in LPS-stimulated alveolar macrophages was obtained in the 
      presence of BHT or NAC. Our data from quantitative reverse 
      transcription-polymerase chain reaction showed that the observed decrease in 
      protein levels of TNF-alpha was associated with a decrease in TNF-alpha mRNA 
      expression. CONCLUSIONS: Our results indicate that antioxidant treatment may be 
      an effective step to lower the inflammatory process caused by cytomegalovirus 
      infection or in endotoxin (LPS)-activated macrophages. The therapeutic use of 
      antioxidant compounds could, therefore, be of interest in conditions such as lung 
      transplantation, in which oxidative stress and inflammation can contribute 
      significantly to the loss of allograft function.
FAU - Hulten, L M
AU  - Hulten LM
AD  - Wallenberg Laboratory, Sahlgrenska University Hospital, Goteborg, Sweden. 
      Lillemor.Mattsson@wlab.wall.gu.SE
FAU - Lindmark, H
AU  - Lindmark H
FAU - Schersten, H
AU  - Schersten H
FAU - Wiklund, O
AU  - Wiklund O
FAU - Nilsson, F N
AU  - Nilsson FN
FAU - Riise, G C
AU  - Riise GC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antioxidants)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1P9D0Z171K (Butylated Hydroxytoluene)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*pharmacology
MH  - Adult
MH  - Antioxidants/*pharmacology
MH  - Butylated Hydroxytoluene/*pharmacology
MH  - Female
MH  - Gene Expression/drug effects
MH  - Graft Survival/drug effects/immunology
MH  - Heart Transplantation/*immunology
MH  - Heart-Lung Transplantation/*immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Lipopolysaccharides/immunology
MH  - Macrophage Activation/drug effects/immunology
MH  - Macrophages, Alveolar/*drug effects/immunology
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/genetics
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
EDAT- 1998/08/29 00:00
MHDA- 1998/08/29 00:01
CRDT- 1998/08/29 00:00
PHST- 1998/08/29 00:00 [pubmed]
PHST- 1998/08/29 00:01 [medline]
PHST- 1998/08/29 00:00 [entrez]
AID - 10.1097/00007890-199808150-00014 [doi]
PST - ppublish
SO  - Transplantation. 1998 Aug 15;66(3):364-9. doi: 10.1097/00007890-199808150-00014.