PMID- 9723690
OWN - NLM
STAT- MEDLINE
DCOM- 19981116
LR  - 20190512
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 10
IP  - 8
DP  - 1998 Aug
TI  - Loss of SDF-1 receptor expression during positive selection in the thymus.
PG  - 1049-56
AB  - SDF-1 is a member of the CXC chemokines. In contrast to other chemokines that are 
      induced by inflammation, SDF-1 is constitutively produced by stromal cells. In 
      order to investigate the physiological roles of SDF-1, we constructed a fusion 
      protein, SDF-1-Cgamma1, composed from murine SDF-1alpha and the constant region 
      of human IgG. SDF-1-Cgamma1 stained EL-4 T lymphoma cells and the staining was 
      blocked by rhSDF-1beta. The expression levels of SDF-1R altered along with the T 
      cell maturation. Most c-kit+ hematopoietic precursors in fetal liver in 
      gestational day (GD) 14.5 embryo were SDF-1R-, while c-kit+ double-negative (DN) 
      thymocytes in the embryo were positive for SDF-1R. The receptor expression 
      increased along with T cell maturation up to double-positive (DP) cell stage. 
      Interestingly, SDF-1R expression was down-modulated after positive selection; 
      CD69+CD3hi DP and CD3hi single-positive thymocytes were SDF-1R-/lo. Northern blot 
      analysis demonstrated that SDF-1 and CXCR4 mRNAs were abundantly expressed in the 
      thymuses of embryo and adult mice. These results demonstrate that SDF-1R 
      expression is involved in T cell development in the thymus, particularly in 
      positive selection.
FAU - Suzuki, G
AU  - Suzuki G
AD  - Division of Radiation Health, National Institute of Radiological Sciences, Chiba, 
      Japan.
FAU - Nakata, Y
AU  - Nakata Y
FAU - Dan, Y
AU  - Dan Y
FAU - Uzawa, A
AU  - Uzawa A
FAU - Nakagawa, K
AU  - Nakagawa K
FAU - Saito, T
AU  - Saito T
FAU - Mita, K
AU  - Mita K
FAU - Shirasawa, T
AU  - Shirasawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Antigens, CD)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Immunoglobulin gamma-Chains)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Animals
MH  - Antigens, CD/immunology
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Blotting, Northern
MH  - Bone Marrow/embryology/immunology
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/genetics/*metabolism
MH  - Down-Regulation
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/immunology
MH  - Humans
MH  - Immunoglobulin gamma-Chains/genetics/*metabolism
MH  - Liver/embryology/immunology
MH  - Lymphoma
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Organ Specificity/genetics
MH  - Proto-Oncogene Proteins c-kit/immunology
MH  - Receptors, CXCR4/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Recombinant Proteins/metabolism
MH  - T-Lymphocyte Subsets/*immunology
MH  - Thymus Gland/embryology/*immunology
MH  - Tumor Cells, Cultured
EDAT- 1998/09/02 00:00
MHDA- 1998/09/02 00:01
CRDT- 1998/09/02 00:00
PHST- 1998/09/02 00:00 [pubmed]
PHST- 1998/09/02 00:01 [medline]
PHST- 1998/09/02 00:00 [entrez]
AID - 10.1093/intimm/10.8.1049 [doi]
PST - ppublish
SO  - Int Immunol. 1998 Aug;10(8):1049-56. doi: 10.1093/intimm/10.8.1049.