PMID- 9723990
OWN - NLM
STAT- MEDLINE
DCOM- 19980903
LR  - 20131121
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 17
IP  - 8
DP  - 1998 Aug
TI  - Binding of Acanthamoeba to [corrected] mannose-glycoproteins of corneal 
      epithelium: effect of injury.
PG  - 770-6
AB  - PURPOSE: Acanthamoeba keratitis is a sight-threatening corneal infection. It is 
      known that: (i) more amoebae bind to the surface of injured corneas than to the 
      normal corneal surface and (ii) mannose-containing glycoproteins (GPs) possess 
      binding sites for Acanthamoeba. The present study was undertaken to determine 
      whether subtle corneal surface injury exposes mannose-GPs and whether more 
      amoebae bind to the mannose-GPs of injured corneas than to those of normal 
      corneas. METHODS: Corneal cup assays were developed to determine whether corneal 
      surface injury exposes binding sites for a mannose/glucose-specific lectin, 
      succinylated-concanavalin A (s-ConA). To determine whether injury exposes 
      mannose-GPs, corneal surface proteins were biotinylated, biotin-labeled 
      mannose-GPs were allowed to bind to s-ConA-agarose beads and were analyzed by 
      SDS-polyacrylamide gel electrophoresis (PAGE). Amoeba binding to mannose-GPs of 
      corneal epithelia was analyzed by PAGE-blot overlay assays. RESULTS: S-ConA 
      binding site density was 2.4 times greater on the injured corneal surface than on 
      the surface of normal corneas. Based on the analysis of the s-ConA-bound, 
      biotin-labeled corneal surface proteins, approximately 5.2 times greater amounts 
      of mannose-GPs were present on the surface of injured corneas than on the normal 
      corneal surface. PAGE-blot overlay assays of s-ConA bound GPs of unlabeled 
      corneal epithelia revealed that, on a per mg total cell protein basis, injured 
      corneal epithelium contained 1.8 times greater amounts of Acanthamoeba-reactive 
      mannose-GPs than normal corneal epithelium. CONCLUSIONS: Subtle corneal injury 
      exposes mannose-GPs on the surface of injured corneas. The newly exposed GPs may 
      serve to provide additional attachment sites for the amoebae. This, in turn, 
      could render the cornea susceptible to the infection.
FAU - Jaison, P L
AU  - Jaison PL
AD  - The New England Eye Center and Department of Ophthalmology, Tufts University 
      School of Medicine, Boston, MA 02111, USA.
FAU - Cao, Z
AU  - Cao Z
FAU - Panjwani, N
AU  - Panjwani N
LA  - eng
GR  - EY07088/EY/NEI NIH HHS/United States
GR  - EY09349/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Membrane Glycoproteins)
RN  - 11028-71-0 (Concanavalin A)
RN  - 55128-23-9 (succinylconcanavalin A)
RN  - PHA4727WTP (Mannose)
SB  - IM
EIN - Curr Eye Res 1998 Oct;17(10):1036
MH  - Acanthamoeba/*physiology
MH  - Adhesiveness
MH  - Animals
MH  - Binding Sites
MH  - Concanavalin A/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epithelium, Corneal/injuries/*parasitology/pathology
MH  - Eye Injuries/metabolism/*parasitology/pathology
MH  - Immunoenzyme Techniques
MH  - Mannose/*metabolism
MH  - Membrane Glycoproteins/*metabolism
MH  - Rabbits
EDAT- 1998/09/02 00:00
MHDA- 1998/09/02 00:01
CRDT- 1998/09/02 00:00
PHST- 1998/09/02 00:00 [pubmed]
PHST- 1998/09/02 00:01 [medline]
PHST- 1998/09/02 00:00 [entrez]
PST - ppublish
SO  - Curr Eye Res. 1998 Aug;17(8):770-6.