PMID- 9724100
OWN - NLM
STAT- MEDLINE
DCOM- 19980908
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 78
IP  - 1
DP  - 1998 Sep 25
TI  - Delineation of the breakpoint at 18q21.1 in a cell line (Karpas1106) derived from 
      mediastinal B-cell lymphoma by fluorescence in situ hybridization with multiple 
      YAC clones.
PG  - 100-5
AB  - The breakpoint of the 18q21 translocation of B-cell-non-Hodgkin's lymphoma (NHL) 
      cell line Karpas1106P was delineated by fluorescence in situ hybridization 
      (FISH). Karpas1106P was derived from mediastinal lymphoblastic B-cell lymphoma 
      and exhibited the immunophenotype characteristic of marginal-zone B-cell lymphoma 
      (MZL): smIg+, pan-B antigen+, CD5-, CD10- and CD23-. The original G-banded 
      karyotype showed a complex translocation containing t(X;18;13)(q28;q21;q12.1). 
      Double-color FISH (DCFISH) with whole-chromosome-painting (WCP) probes for 
      chromosomes X, 13 and 18, and 18q-specific yeast artificial chromosome (YAC) 
      clones defined t(X;18;13) as ider(X)t(X;18; 13)(q28;q 12.3q21.1;q12.1). The 
      immunoglobulin-heavy-chain (IgH) gene was not involved in the chromosomal 
      translocation as detected by DCFISH with VH and Cgamma probes. By using 
      contiguous YAC clones mapped from 18q12.3 to q21.1, we identified a YAC clone 
      y852H2 with its breakpoint at 18q21.1. In Karpas1106P, the distal part of 
      chromosome 18 from the breakpoint (18q21.1-qter) was deleted, showing loss of 
      heterozygosity of this region. In addition, the chromosomal segment 18q21.1 was 
      duplicated and inserted to ider(X)t(X;18; 13) between Xq28 and 13q12.1 with 
      maintaining its original orientation. The DNA sequence of the breakpoint region 
      contained in y852H2 can serve as a candidate locus for further molecular 
      dissection to identify the causative gene of MZL.
FAU - Tamura, A
AU  - Tamura A
AD  - Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Akagi, T
AU  - Akagi T
FAU - Nakazawa, N
AU  - Nakazawa N
FAU - Kashima, K
AU  - Kashima K
FAU - Nakamura, S
AU  - Nakamura S
FAU - Karpas, A
AU  - Karpas A
FAU - Silverman, G A
AU  - Silverman GA
FAU - Morishima, Y
AU  - Morishima Y
FAU - Taniwaki, M
AU  - Taniwaki M
FAU - Seto, M
AU  - Seto M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
MH  - X Chromosome/genetics
EDAT- 1998/09/02 05:27
MHDA- 2000/06/20 09:00
CRDT- 1998/09/02 05:27
PHST- 1998/09/02 05:27 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/09/02 05:27 [entrez]
AID - 10.1002/(SICI)1097-0215(19980925)78:1<100::AID-IJC16>3.0.CO;2-F [pii]
AID - 10.1002/(sici)1097-0215(19980925)78:1<100::aid-ijc16>3.0.co;2-f [doi]
PST - ppublish
SO  - Int J Cancer. 1998 Sep 25;78(1):100-5. doi: 
      10.1002/(sici)1097-0215(19980925)78:1<100::aid-ijc16>3.0.co;2-f.