PMID- 9724455
OWN - NLM
STAT- MEDLINE
DCOM- 19981116
LR  - 20190720
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 294
IP  - 1
DP  - 1998 Oct
TI  - Enkephalin-immunoreactive subpopulations in the myenteric plexus of the 
      guinea-pig fundus project primarily to the muscle and not to the mucosa.
PG  - 45-55
AB  - Enkephalin (ENK) immunoreactivity was localised in different neuronal 
      subpopulations of the myenteric plexus in the guinea-pig gastric fundus using 
      immunohistochemistry for neurone-specific enolase (NSE), ENK, choline 
      acetyltransferase (ChAT), substance P (SP), neuropeptide Y (NPY), calretinin 
      (CALRET), and somatostatin (SOM). NADPH-diaphorase staining was used to label 
      nitric oxide synthase (NOS)-containing neurones. ENK was observed in 44% of the 
      myenteric neurones. The major ENK-positive subpopulations were ChAT/ENK (35% of 
      ENK-positive neurones), ChAT/SP/ENK (26%), NOS/NPY/ENK (22%) and 
      ChAT/SP/ENK/CALRET (9%). The projection pathways of these ENK-positive 
      subpopulations to the circular muscle and the mucosa were determined using 
      retrograde labelling with DiI in organ culture followed by immunohistochemistry. 
      Of myenteric neurones retrogradely labelled from the mucosa and the circular 
      muscle, 13% and 48% exhibited ENK immunoreactivity, respectively. Three major 
      ENK-positive subpopulations innervating the mucosa or circular muscle were 
      identified: ascending ChAT/SP/ENK (7% of all mucosa neurones; 24% of all circular 
      muscle neurones), ascending ChAT/ENK (4%; 15%) and descending NOS/NPY/ENK (1%; 
      8%) neurones. Only very few CALRET- or SOM-positive neurones projected to the 
      mucosa or circular muscle. ChAT/SP/ENK and ChAT/ENK neurones might function as 
      ascending excitatory muscle motor neurones, whereas NOS/NPY/ENK neurones are most 
      likely descending inhibitory muscle motor neurones. The relatively few 
      ENK-positive mucosa neurones do not favour a major involvement of ENK-positive 
      myenteric neurones in the control of gastric mucosa activity.
FAU - Pfannkuche, H
AU  - Pfannkuche H
AD  - Physiologisches Institut, Tierarztliche Hochschule, Bischofsholer Damm 15/102, 
      D-30173 Hannover, Germany.
FAU - Reiche, D
AU  - Reiche D
FAU - Firzlaff, U
AU  - Firzlaff U
FAU - Sann, H
AU  - Sann H
FAU - Schemann, M
AU  - Schemann M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Calbindin 2)
RN  - 0 (Carbocyanines)
RN  - 0 (Enkephalins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Neuropeptide Y)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 33507-63-0 (Substance P)
RN  - 51110-01-1 (Somatostatin)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
SB  - IM
MH  - Animals
MH  - Calbindin 2
MH  - Carbocyanines
MH  - Choline O-Acetyltransferase/metabolism
MH  - Enkephalins/*metabolism
MH  - Fluorescent Dyes
MH  - Gastric Fundus/*innervation
MH  - Gastric Mucosa/innervation
MH  - Guinea Pigs
MH  - Immunohistochemistry
MH  - Motor Neurons/metabolism
MH  - Muscle, Smooth/innervation
MH  - Myenteric Plexus/cytology/*metabolism
MH  - Neural Pathways/cytology/metabolism
MH  - Neurons/classification/metabolism
MH  - Neuropeptide Y/metabolism
MH  - S100 Calcium Binding Protein G/metabolism
MH  - Somatostatin/metabolism
MH  - Substance P/metabolism
EDAT- 1998/09/02 00:00
MHDA- 1998/09/02 00:01
CRDT- 1998/09/02 00:00
PHST- 1998/09/02 00:00 [pubmed]
PHST- 1998/09/02 00:01 [medline]
PHST- 1998/09/02 00:00 [entrez]
AID - 10.1007/s004410051155 [doi]
PST - ppublish
SO  - Cell Tissue Res. 1998 Oct;294(1):45-55. doi: 10.1007/s004410051155.