PMID- 9725554
OWN - NLM
STAT- MEDLINE
DCOM- 19981214
LR  - 20071115
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 33
IP  - 1
DP  - 1998 Sep 1
TI  - Advances in the automated detection of metaphase chromosomes labeled with 
      fluorescence dyes.
PG  - 10-8
AB  - Applications of fluorescence in situ hybridization (FISH) for translocation 
      studies and biological dosimetry would benefit substantially from reliable and 
      efficient automatic detection of metaphase chromosomes labeled with fluorescent 
      dyes. We replicated and evaluated a fluorescence metaphase finder previously 
      developed at the Medical Research Council (MRC), Human Genetics Unit (Scotland) 
      and at Lawrence Berkeley Laboratory (LBL; California). The MRC/LBL system seemed 
      to detect nearly all of the metaphases on the test slides, but it presented an 
      unacceptable number of false positives (about five false positives per one true 
      positive). Furthermore, we determined that the system actually overcalled true 
      detections by counting certain metaphase spreads twice (duplicates). Through 
      modifications of the MRC/LBL system, we developed the Lawrence Livermore National 
      Laboratory (LLNL) system, which minimizes the detection of duplicates, 
      incorporates new detection features, uses a binary decision tree (BDT) for 
      classification, and provides functionalities to improve scanning accuracy and 
      improve the post-detection review. To test the new system, DAPI-stained 
      preparations of metaphase chromosomes from blood lymphocytes of four unrelated 
      donors were placed on slides in drops ranging from 7 mm to 20 mm in diameter. 
      Drops contained between 5 and 200 scorable metaphases each. The LLNL system 
      achieved approximately 90% detection of non-duplicated metaphases as verified by 
      an expert cytogeneticist, with typically less than one false positive per every 
      one true positive detected.
FAU - Mascio, L N
AU  - Mascio LN
AD  - Defense Sciences Engineering Division, Lawrence Livermore National Laboratory, 
      Livermore, California 94551, USA.
FAU - Yuen, B K
AU  - Yuen BK
FAU - Kegelmeyer, W P Jr
AU  - Kegelmeyer WP Jr
FAU - Matsumoto, K
AU  - Matsumoto K
FAU - Briner, J
AU  - Briner J
FAU - Wyrobek, A J
AU  - Wyrobek AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Automation
MH  - Cells, Cultured
MH  - *Chromosomes, Human
MH  - Evaluation Studies as Topic
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Metaphase
MH  - Sensitivity and Specificity
EDAT- 1998/09/02 05:29
MHDA- 2000/06/20 09:00
CRDT- 1998/09/02 05:29
PHST- 1998/09/02 05:29 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/09/02 05:29 [entrez]
AID - 10.1002/(SICI)1097-0320(19980901)33:1<10::AID-CYTO2>3.0.CO;2-F [pii]
PST - ppublish
SO  - Cytometry. 1998 Sep 1;33(1):10-8.