PMID- 9726900
OWN - NLM
STAT- MEDLINE
DCOM- 19981030
LR  - 20200724
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 64
IP  - 9
DP  - 1998 Sep
TI  - Identification and activities in situ of Nitrosospira and Nitrospira spp. as 
      dominant populations in a nitrifying fluidized bed reactor.
PG  - 3480-5
AB  - Bacterial aggregates from a chemolithoautotrophic, nitrifying fluidized bed 
      reactor were investigated with microsensors and rRNA-based molecular techniques. 
      The microprofiles of O2, NH4+, NO2-, and NO3- demonstrated the occurrence of 
      complete nitrification in the outer 125 microgram of the aggregates. The ammonia 
      oxidizers were identified as members of the Nitrosospira group by fluorescence in 
      situ hybridization (FISH). No ammonia- or nitrite-oxidizing bacteria of the genus 
      Nitrosomonas or Nitrobacter, respectively, could be detected by FISH. To identify 
      the nitrite oxidizers, a 16S ribosomal DNA clone library was constructed and 
      screened by denaturing gradient gel electrophoresis and selected clones were 
      sequenced. The organisms represented by these sequences formed two 
      phylogenetically distinct clusters affiliated with the nitrite oxidizer 
      Nitrospira moscoviensis. 16S rRNA-targeted oligonucleotide probes were designed 
      for in situ detection of these organisms. FISH analysis showed that the dominant 
      populations of Nitrospira spp. and Nitrosospira spp. formed separate, dense 
      clusters which were in contact with each other and occurred throughout the 
      aggregate. A second, smaller, morphologically and genetically different 
      population of Nitrospira spp. was restricted to the outer nitrifying zones.
FAU - Schramm, A
AU  - Schramm A
AD  - Max-Planck-Institut fur Marine Mikrobiologie, D-28359 Bremen, Germany. 
      aschramm@mpibremen.de
FAU - De Beer, D
AU  - De Beer D
FAU - Wagner, M
AU  - Wagner M
FAU - Amann, R
AU  - Amann R
LA  - eng
SI  - GENBANK/AJ224038
SI  - GENBANK/AJ224039
SI  - GENBANK/AJ224040
SI  - GENBANK/AJ224041
SI  - GENBANK/AJ224042
SI  - GENBANK/AJ224043
SI  - GENBANK/AJ224044
SI  - GENBANK/AJ224045
SI  - GENBANK/AJ224046
SI  - GENBANK/AJ224047
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/*metabolism
MH  - *Bioreactors
MH  - DNA, Ribosomal
MH  - Fresh Water
MH  - Gene Library
MH  - Gram-Negative Chemolithotrophic Bacteria/*classification/genetics/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Nitrates/metabolism
MH  - Nitrites/*metabolism
MH  - Oligonucleotide Probes
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
PMC - PMC106750
EDAT- 1998/09/03 00:00
MHDA- 1998/09/03 00:01
PMCR- 1998/09/01
CRDT- 1998/09/03 00:00
PHST- 1998/09/03 00:00 [pubmed]
PHST- 1998/09/03 00:01 [medline]
PHST- 1998/09/03 00:00 [entrez]
PHST- 1998/09/01 00:00 [pmc-release]
AID - 0153 [pii]
AID - 10.1128/AEM.64.9.3480-3485.1998 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1998 Sep;64(9):3480-5. doi: 
      10.1128/AEM.64.9.3480-3485.1998.