PMID- 9726955
OWN - NLM
STAT- MEDLINE
DCOM- 19981130
LR  - 20181113
IS  - 0006-3495 (Print)
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Linking)
VI  - 75
IP  - 3
DP  - 1998 Sep
TI  - Simulating the role of microtubules in depolymerization-driven transport: a Monte 
      Carlo approach.
PG  - 1529-40
AB  - In this paper we present a model that simulates the role of microtubules in 
      depolymerization-driven transport. The model simulates a system that consists of 
      a 13-protofilament microtubule with "five-start" helical structure and a motor 
      protein-coated bead that moves along one of the protofilaments of the 
      microtubule, as in in vitro experiments. The microtubule is simulated using the 
      lateral cap model, with substantial generalizations. For the new terminal 
      configurations in the presence of the bead, rate constants for association and 
      dissociation events of tubulin molecules are calculated by exploring the 
      geometric similarities between different patterns of terminal configurations and 
      by decomposing complex patterns into simpler patterns whose corresponding rate 
      constants are known. In comparison with a previous model, in which 
      simplifications are made about the structure of the microtubule and in which the 
      microtubule can only depolymerize, the detailed structure of the microtubule is 
      taken into account in the present model. Furthermore, the microtubule can be 
      either polymerizing or depolymerizing. Force-velocity curves are obtained for 
      both zero and non-zero tubulin guanosine 5'-triphosphate (GTP) concentrations. By 
      analyzing the trajectory of the bead under different parameters, the condition 
      for "run and pause" is analyzed, and the time scale of "run" and "pause" is found 
      to be different for different motor proteins. We also suggest experiments that 
      can be used to examine the results predicted by the model.
FAU - Tao, Y C
AU  - Tao YC
AD  - Department of Physics, New York University, New York 10003, USA.
FAU - Peskin, C S
AU  - Peskin CS
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Biopolymers)
RN  - 0 (Molecular Motor Proteins)
RN  - 0 (Tubulin)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/physiology
MH  - Biological Transport, Active
MH  - Biophysical Phenomena
MH  - Biophysics
MH  - Biopolymers/chemistry/physiology
MH  - Guanosine Triphosphate/physiology
MH  - Kinetics
MH  - Microtubules/chemistry/*physiology
MH  - *Models, Biological
MH  - Models, Molecular
MH  - Molecular Motor Proteins/chemistry/*physiology
MH  - Monte Carlo Method
MH  - Protein Conformation
MH  - Tubulin/chemistry/physiology
PMC - PMC1299828
EDAT- 1998/09/03 00:00
MHDA- 1998/09/03 00:01
PMCR- 1999/09/01
CRDT- 1998/09/03 00:00
PHST- 1998/09/03 00:00 [pubmed]
PHST- 1998/09/03 00:01 [medline]
PHST- 1998/09/03 00:00 [entrez]
PHST- 1999/09/01 00:00 [pmc-release]
AID - S0006-3495(98)74072-X [pii]
AID - 10.1016/S0006-3495(98)74072-X [doi]
PST - ppublish
SO  - Biophys J. 1998 Sep;75(3):1529-40. doi: 10.1016/S0006-3495(98)74072-X.