PMID- 9728612
OWN - NLM
STAT- MEDLINE
DCOM- 19980916
LR  - 20190512
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 110
IP  - 3
DP  - 1998 Sep
TI  - A comprehensive system to explore p53 mutations.
PG  - 368-73
AB  - To establish an effective and reliable system for the detection of p53 mutations, 
      we evaluated the detection efficiencies of nonisotopic polymerase chain 
      reaction-single-strand conformation polymorphism (PCR-SSCP), fluorescence in situ 
      hybridization (FISH), and immunohistochemistry. Ten cell lines (AsPc1, BxPc3, 
      Miapaca2, Panc1, Colo320-011, Lovo, MCF7, LNCaP, HL-60, and Daudi), a peripheral 
      blood sample from a patient with a p53 germline mutation (p53GML), and a normal 
      peripheral blood sample were used for examination. Direct nucleotide sequencing 
      identified p53 mutations in 7 of 12 samples (AsPc1, BxPc3, Miapaca2, Panc1, 
      Colo320-011, HL-60, and p53GML). The nonisotopic PCR-SSCP detected anomalies of 
      the PCR fragments in 5 cell lines. In the FISH analysis, 2 cell lines exhibited 
      loss of heterozygosity of the p53 locus. Immunohistochemistry detected an 
      accumulation of the abnormal p53 in 4 cell lines. The combination of these 3 
      methods produced no false-negative or false-positive results. This combination 
      may be an excellent and beneficial system for the clinical diagnosis of the 
      various human cancers.
FAU - Furuwatari, C
AU  - Furuwatari C
AD  - Central Clinical Laboratories, Shinshu University Hospital, Matsumoto, Japan.
FAU - Yagi, A
AU  - Yagi A
FAU - Yamagami, O
AU  - Yamagami O
FAU - Ishikawa, M
AU  - Ishikawa M
FAU - Hidaka, E
AU  - Hidaka E
FAU - Ueno, I
AU  - Ueno I
FAU - Furihata, K
AU  - Furihata K
FAU - Ogiso, Y
AU  - Ogiso Y
FAU - Katsuyama, T
AU  - Katsuyama T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Cell Line
MH  - DNA Mutational Analysis
MH  - DNA, Neoplasm/*analysis
MH  - Evaluation Studies as Topic
MH  - False Positive Reactions
MH  - *Genes, p53/*genetics
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity
MH  - *Mutation
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - Sequence Analysis, DNA
MH  - Tumor Suppressor Protein p53/*genetics/metabolism
EDAT- 1998/09/05 00:00
MHDA- 1998/09/05 00:01
CRDT- 1998/09/05 00:00
PHST- 1998/09/05 00:00 [pubmed]
PHST- 1998/09/05 00:01 [medline]
PHST- 1998/09/05 00:00 [entrez]
AID - 10.1093/ajcp/110.3.368 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 1998 Sep;110(3):368-73. doi: 10.1093/ajcp/110.3.368.