PMID- 9729350
OWN - NLM
STAT- MEDLINE
DCOM- 19981002
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 404
IP  - 1-2
DP  - 1998 Aug 3
TI  - An automated new technique for scoring the rodent micronucleus assay: 
      computerized image analysis of acridine orange supravitally stained peripheral 
      blood cells.
PG  - 149-54
AB  - We developed an automated image analysis system to obtain objective data for the 
      rodent peripheral blood micronucleus assay with acridine orange (AO) supravital 
      staining. The system was able to identify micronucleated reticulocytes (MNRETs) 
      and to evaluate inhibition of bone marrow cell proliferation by measuring the 
      reticular area of reticulocytes (RETs). We also developed automated equipment to 
      produce homogeneous acridine orange-coated glass slides. This study was designed 
      to compare automated scoring with manual scoring using 4 model clastogens and 2 
      mouse strains. The MNRET incidence induced by each clastogen was similar for 
      automated and manual scoring, and there was good correlation (r=0.92) between the 
      methods. In addition, an index of bone marrow toxicity based on the reticular 
      area of RETs was compared to the conventional index (% of polychromatic 
      erythrocytes (PCE) to total erythrocytes; PCE ratio) and was similar. The results 
      indicated that our technique for computer-assisted image analysis for the 
      micronucleus assay with AO supravitally stained peripheral blood RETs was 
      comparable to conventional microscopic scoring, and it was superior in 
      objectivity and statistical power.
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Asano, N
AU  - Asano N
AD  - Toxicological Research Division, Nitto Denko Corporation, 1-1-2, Shimohozumi, 
      Ibaraki, Osaka 567-8680, Japan. asanonri@nitto.co.jp
FAU - Katsuma, Y
AU  - Katsuma Y
FAU - Tamura, H
AU  - Tamura H
FAU - Higashikuni, N
AU  - Higashikuni N
FAU - Hayashi, M
AU  - Hayashi M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*metabolism
MH  - Animals
MH  - Automation
MH  - Bone Marrow Cells/cytology
MH  - Cell Division/drug effects
MH  - Image Processing, Computer-Assisted
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Micronucleus Tests/*methods
MH  - Microscopy
MH  - Mutagens/pharmacology
MH  - Particle Size
MH  - Reticulocytes/cytology
EDAT- 1998/09/05 00:00
MHDA- 1998/09/05 00:01
CRDT- 1998/09/05 00:00
PHST- 1998/09/05 00:00 [pubmed]
PHST- 1998/09/05 00:01 [medline]
PHST- 1998/09/05 00:00 [entrez]
AID - S0027-5107(98)00108-0 [pii]
AID - 10.1016/s0027-5107(98)00108-0 [doi]
PST - ppublish
SO  - Mutat Res. 1998 Aug 3;404(1-2):149-54. doi: 10.1016/s0027-5107(98)00108-0.