PMID- 9731218
OWN - NLM
STAT- MEDLINE
DCOM- 19981002
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 249
IP  - 3
DP  - 1998 Aug 28
TI  - Regulation of the Drosophila bHLH-PAS protein Sima by hypoxia: functional 
      evidence for homology with mammalian HIF-1 alpha.
PG  - 811-6
AB  - Hypoxia inducible factor-1 (HIF-1) is a heterodimeric complex of two 
      basic-helix-loop-helix proteins of the PAS family which is critical for 
      oxygen-dependent expression of many mammalian genes. Regulation is mediated by 
      the alpha subunit (HIF-1 alpha) and sequences from HIF-1 alpha can confer 
      hypoxia-inducible activity on a Ga14 fusion protein. To analyse conservation of 
      this system of gene regulation between Drosophila and mammalian cells we 
      constructed Ga14 fusions with a series of Drosophila basic-helix-loop-helix PAS 
      (bHLH-PAS) proteins and tested for hypoxia inducibility in transfected Hep3B 
      cells. We found that Ga14 functions with Similar (Sima) but not other Drosophila 
      bHLH-PAS proteins showed inducible activity following exposure to stimuli which 
      classically activate mammalian HIF-1:hypoxia, cobaltous ions, and 
      desferrioxamine. We also found that Sima protein accumulated in Drosophila SL2 
      cells following hypoxia. Together these findings indicate the existence of 
      functional homologies between Sima and HIF-1 alpha, and that conservation is such 
      as to enable Sima to interact with the hypoxia signal transduction system in 
      mammalian cells.
FAU - Bacon, N C
AU  - Bacon NC
AD  - Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, 
      United Kingdom.
FAU - Wappner, P
AU  - Wappner P
FAU - O'Rourke, J F
AU  - O'Rourke JF
FAU - Bartlett, S M
AU  - Bartlett SM
FAU - Shilo, B
AU  - Shilo B
FAU - Pugh, C W
AU  - Pugh CW
FAU - Ratcliffe, P J
AU  - Ratcliffe PJ
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Insect Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Sima protein, Drosophila)
RN  - 0 (Transcription Factors)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Cell Hypoxia/genetics/physiology
MH  - Cell Line
MH  - DNA-Binding Proteins/chemistry/*genetics/metabolism
MH  - Drosophila/*genetics/metabolism
MH  - *Drosophila Proteins
MH  - Gene Expression Regulation
MH  - Genes, Insect
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Insect Proteins/chemistry/*genetics/metabolism
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Oxygen/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Signal Transduction
MH  - Species Specificity
MH  - *Transcription Factors
MH  - Transcriptional Activation
MH  - Transfection
EDAT- 1998/09/10 00:00
MHDA- 1998/09/10 00:01
CRDT- 1998/09/10 00:00
PHST- 1998/09/10 00:00 [pubmed]
PHST- 1998/09/10 00:01 [medline]
PHST- 1998/09/10 00:00 [entrez]
AID - S0006-291X(98)99234-8 [pii]
AID - 10.1006/bbrc.1998.9234 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1998 Aug 28;249(3):811-6. doi: 
      10.1006/bbrc.1998.9234.